超高效液相色谱法检测香菇菌多糖片单糖组分

Determination of monosaccharide compositions in Lentinus edodes polysaccharide tablets by ultra performance liquid chromatography

中文关键词:  香菇菌多糖片  单糖组分  超高效液相色谱法  甘露糖  葡萄糖  半乳糖

英文关键词:Lentinus edodes polysaccharide tablets  monosaccharide composition  ultra performance liquid chromatography  D-mannose  D-glucose  D-galactose

中文摘要:

      目的 建立一种柱前衍生-超高效液相色谱法(ultra performance liquid chromatography, UPLC)检测香菇菌多糖片中单糖组成的分析方法。方法 为排除香菇菌多糖片中辅料干扰, 采用UPLC对香菇菌多糖原料中单糖组分进行定性分析, 确定原料中的单糖组成。对超高效液相色谱法进行优化及方法学考察, 以香菇菌多糖原料中主要单糖组成为依据对香菇菌多糖片单糖组成进行分析, 确定香菇菌多糖片的单糖组成及摩尔比例。结果 香菇菌多糖片的主要糖单元为甘露糖、葡萄糖和半乳糖, 摩尔比为: 4.6:4.28:6.375, 方法学验证结果表明该单糖组成测定方法的精密度良好, 样品中所测单糖的加样回收率为96.6%~103.8%, 相对标准偏差在2.0%~2.6%之间, 各单糖标准曲线线性相关系数大于0.999, 线性关系良好。结论 此方法快速、高效、准确, 并为香菇菌多糖片的质量控制提供了保障。

英文摘要:

      Objective To establish a pre-column derivatization method for determination of monosaccharide compositions in Lentinus edodes polysaccharide tables by ultra performance liquid chromatography (UPLC). Methods In order to eliminate the interference in the compound of Lentinus edodes polysaccharide tables, the monosaccharide compositions were determined by UPLC. Qualitative analysis was used on the monosaccharide components of Lentinus edodes polysaccharide raw materials and the monosaccharide was analyzed in raw materials. The optimization and methodology of UPLC were investigated. The main monosaccharide components of Lentinus edodes polysaccharide raw materials were analyzed. The proportions of monosaccharide and molar ratios of polysaccharides were determined. Results The monosaccharide compositions of Lentinus edodes polysaccharide tablet were D-mannose, D-glucose and D-galactose by UPLC. The molar ratios of monosaccharides (D-mannose:D-glucose:D-galactose) in Lentinus edodes polysaccharide tablet were 4.6:4.28:6.375, respectively. The recoveries of these monosaccharides were 96.6%~103.8% and relative standard deviations were 2.0%~2.6%. The standard curve of each monosaccharide exhibited a good linear correlation with the coefficient higher than 0.999. Conclusion The established method is rapid, efficient and accurate. It also provides the quality control for Lentinus edodes polysaccharide tablet.