自上而下法评定固相酶联免疫吸附法测定食用油中黄曲霉毒素B1的不确定度

Uncertainty evaluation of determination of aflatoxin B1 in edible oil by ELISA with top-down approach

中文关键词:  固相酶联免疫吸附法  黄曲霉毒素B1  不确定度  自上而下法  食用油

英文关键词:solid-phase enzyme-linked immunosorbent assay  aflatoxin B1  uncertainty  top-down approach  edible oil

中文摘要:

      目的 利用自上而下方法评定固相酶联免疫吸附法测定食用油中黄曲霉毒素B1含量的不确定度。 方法 在控制不确定度来源或程序的前提下, 得出试验数据、质控数据进行评定, 通过计算偏移和实验室内复现性2个分量, 最后合成得到该方法的测量不确定度。结果 按照自上而下法的公式计算, 本研究测得食物油中黄曲霉毒素B1含量为(46.25±6.28) μg/mL, 扩展不确定度Urel为14%(k=2)。结论 使用自上而下法对固相酶联免疫吸附法测定食用油中黄曲霉毒素B1含量进评定过程是实用和可靠的, 可以为其他同类型的检测方法进行测量不确定度评定时所借鉴, 也为实验员在实验质量控制方面提供参考。

英文摘要:

      Objective To determine the uncertainty of aflatoxin B1 in edible oil by solid-phase enzyme-linked immunosorbent assay (ELISA) using top-down approach. Methods The test data and quality control data were evaluated under the premise of controlling the source or procedure of the uncertainty. By calculating the 2 components of offset and laboratory reproducibility, the measurement uncertainty of the method was finally obtained. Results According to the method of top-down approach, the content of aflatoxin B1 in edible oil in this study was (46.25±6.28) μg/mL and the accompanying expanded uncertainty was 14% (k=2). Conclusion It is practical and reliable to determine the content of aflatoxin B1 in edible oil by solid-phase ELISA with top-down approach. It can be used for other non-deterministic measurement of the same type of detection method, and provide a reference for the experimental staff in experimental quality control.