荞麦过敏原致敏蛋白组分研究

Study on allergens of buckwheat allergy

中文关键词:  荞麦  过敏原  致敏蛋白  免疫印迹法

英文关键词:buckwheat  allergen  allergenic protein  immunoblotting

中文摘要:

      目的 对荞麦过敏原的主要致敏蛋白进行研究。方法 收集26例荞麦过敏症患者, 详细询问临床病史, 留取血清建立血清库; 荧光免疫分析仪ImmunoCAP系统检测荞麦特异性IgE水平; 用免疫印迹法测定每种致敏蛋白组分的灰度值。结果 共收集荞麦过敏患者26例(男10例、女16例, 平均年龄29岁)。荞麦特异性IgE的中位数水平是8.385 KUA/L。免疫印迹法识别了荞麦过敏原中的15种致敏蛋白:10 kDa(8%)、13 kDa(4%)、14 kDa(12%)、16 kDa(4%)、18~19 kDa(100%)、22~24 kDa(46%)、34 kDa(31%)、36 kDa(58%)、38 kDa(15%)、40~50 kDa(46%)、54 kDa(23%)、56 kDa(19%)、69~70 kDa(46%)、100~130 kDa(27%)和130~170 kDa(19%)。结论 荞麦中的18~19、22~24、36、40~50和69~70 kDa蛋白显示高阳性率, 可能为荞麦过敏原的主要致敏蛋白。

英文摘要:

      Objective To investigate the most related allergen components in buckwheat allergen. Methods Twenty six patients with buckwheat allergy were enrolled, their detail clinical history was inquired and a serum library was established. Buckwheat specificIgE was checked by the immunoCAP system and immunoblotting was performed to measure intensity of each component. Results Twenty-six patients (10 males and 16 females) were included in this study (average age was 29 years old). The median of buckwheat sIgE was 8.385 KUA/L. Fifteen allergens were identified by immunoblotting: 10 kDa(8%), 13 kDa(4%), 14 kDa(12%), 16 kDa(4%), 18~19 kDa(100%), 22~24 kDa(46%), 34 kDa(31%), 36 kDa(58%), 38 kDa(15%), 40~50 kDa(46%), 54 kDa(23%), 56 kDa(19%), 69~70 kDa(46%), 100~130 kDa(27%) and 130~170 kDa(19%) in the buckwheat proteins. Conclusion The 18~19, 22~24, 36, 40~50 and 69~70 kDa proteins in buckwheat are implicated as major allergens with high positive rates relatively.