苯酚-硫酸法测定三七枸杞酒中多糖含量

Determination of polysaccharide in the alcoholic drink of SanqiGouqi by phenol-sulfuric acid colorimetry method

中文关键词:  多糖  三七枸杞酒  苯酚-硫酸法  含量测定

英文关键词:polysaccharide  alcoholic drink of SanqiGouqi  phenol-vitriolic colorimetry  content determination

中文摘要:

      目的 建立三七枸杞酒中多糖含量的测定方法。方法 三七枸杞酒中的多糖经苯酚-硫酸法显色后, 利用紫外-可见分光光度计在490 nm波长处进行比色测定。结果 苯酚-硫酸法测定三七枸杞酒中多糖方法稳定, 精密度高, 线性范围为0.02~0.1 mg/mL, 相关系数为r2=0.9998, 平均回收率为99.89%。结论 苯酚-硫酸法可作为三七枸杞酒中多糖含量测定的方法。

英文摘要:

      Objective To establish a method for determination of polysaccharide content in the alcoholic drink of SanqiGouqi. Methods The phenol-sulfuric acid colorimetry method was performed at wavelength of 490 nm with phenol-sulfuric acid as a color reagent. Results The phenol-sulfuric acid colorimetry method had stability and high precision for determination of polysaccharide in the alcoholic drink of SanqiGouqi. The good positive linear relationship was shown in the range of 0.02~0.1 mg/mL, the correlation coefficient (r2) was 0.9998, and the average recovery rate of added samples was 99.89%. Conclusion The established method is suitable for determination of polysaccharide in the alcoholic drink of SanqiGouqi.