时间分辨荧光免疫法检测食品中黄曲霉毒素B1的含量

Determination of aflatoxin B1 in food by time-resolved fluoroimunoassay

中文关键词:  黄曲霉毒素B1  时间分辨荧光免疫法  食品

英文关键词:aflatoxin B1  time-resolved fluoroimunoassay  food

中文摘要:

      目的 建立高灵敏度的时间分辨免疫法检测黄曲霉毒素B1的含量。方法 通过双功能螯合剂异氰酸苄基二乙烯三胺四乙酸络合稀土元素Eu3+标记抗AFB1的单克隆抗体, 以AFB1-OVA为固相抗原, 优化反应条件, 建立直接竞争时间分辨免疫检测方法。结果 优化条件后, 方法灵敏度为0.02 μg/L, IC50为0.73 μg/L, 线性检测范围为0.01~30 μg/L, 大米、花生、黄豆和干果样品回收率在91%~104%之间, 检测结果准确; 与结构类似物AFB2、AFG1、AFG2、AFM1的交叉反应率分别为17.3%、4.49%、2.37%和0.73%。结论 本方法灵敏度高、特异性强、检测快速, 可以满足实际样品的检测需求。

英文摘要:

      Objective To establish a hypersensitive time-resolved fluoroimmunoassay (TRFIA) method for detection of aflatoxin B1 (AFB1). Methods The antibody for anti-AFB1 was labeled by Eu3+-DTTA, AFB1-OVA was bounded on the surface of a microtiter plate, the reaction condition was optimized and then the direct competition with TRFIA was established. Results Under optimized assay conditions, the limit of detection was 0.02 μg/L, the IC50 of TRFIA was 0.73 μg/L, the linear detection range was 0.01~30 μg/L, the recoveries for rice, peanuts, soybeans and preserved fruits were range from 91% to 104%, which showed the results of detection were accuracy; and the cross reaction with AFB2, AFG1, AFG2 and AFM1 were 17.3%, 4.49%, 2.37% and 0.73%, respectively. Conclusion The established method is sensitive, specific and rapid, which can meet the detection demand of actual samples.