响应曲面法优化微波辅助提取香菇柄多糖及其 抗氧化活性研究

Optimization of microwave-assisted extraction of polysaccharides from Lentinus edods root by response surface analysis and its antioxidant activity

中文关键词:  香菇柄多糖  微波辅助提取  响应面分析  抗氧化活性

英文关键词:polysaccharide from Lentinus edods root  microwave-assisted extraction  response surface analysis  antioxidant activity

中文摘要:

      目的 优化香菇柄多糖的微波辅助提取工艺, 并研究其抗氧化活性。方法 通过单因素试验, 选择时间、功率以及料液比为自变量, 多糖提取率为响应值, 采用响应曲面法设计分析研究各自变量及其交互作用对多糖提取率的影响。经分析模拟得到二次多项式回归方程的预测模型, 从而获得最适的提取工艺条件; 并采用DPPH法、Fenton反应等方法测定香菇柄多糖的抗氧化活性。结果 在提取时间8 min、微波作用功率400 W、料液比1:7(m:V)的条件下获得多糖提取率为4.91%; 香菇柄多糖具有清除DPPH自由基与羟自由基的能力。 结论 本研究可为香菇柄的再利用与开发提供参考。

英文摘要:

      Objective To optimize the microwave-assisted extraction (MAE) process of polysaccharide from Lentinus edods root (LRP), and study its antioxidant activity. Methods Single factor experiments involving three technological parameters including extraction time, microwave power and material/solvent ratio were selected for following investigations on the interactive effects of the parameters on LRP yield using central composite design combined with response surface methodology. A quadratic polynomial regression model was developed for LRP yield as a response to the above parameters and then the optimal extraction process conditions were obtained. The methods of DPPH and Fenton reaction were applied for determination of antioxidant activity. Results The optimal conditions of MAE were as follows: extraction time was 8 min, microwave power was 400 W and material/solvent ratio was 1:7 (m:V), and the yield of LRP was up to 4.91%. The antioxidant assays showed that the DPPH and the hydroxyl radical scavenging activities of extracts were strong. Conclusion The present study can provide a reference for reuse and development of LRP.