高效液相色谱法测定番茄中腐霉利和异菌脲的 残留量

Determination of procymidone and iprodione residues in tomato by high performance liquid chromatography

中文关键词:  高效液相色谱法  番茄  腐霉利  异菌脲  残留量

英文关键词:high performance liquid chromatography  tomato  procymidone  iprodione  residue

中文摘要:

      目的 建立固相萃取-高效液相色谱法测定番茄中腐霉利和异菌脲的残留量。方法 番茄样品用乙腈超声提取, 加入氯化钠进行盐析脱水, 提取液过Florisil柱净化, 用正己烷/丙酮(90:10, V:V)淋洗液洗脱, 洗脱液氮吹近干, 甲醇定容后经ZORBAX Eclipse XDB-C18(250 mm × 4.6 mm, 5 μm)色谱柱进行分离, 以乙腈/水(60:40, V:V)为流动相, 流速为1.0 mL/min, 采用二极管阵列检测器检测, 检测波长为208 nm。结果 在0.05~5.0 mg/L浓度范围内, 腐霉利和异菌脲的质量浓度与其峰面积呈良好的线性关系(r2 >0.999), 方法检出限均为0.025 mg/kg, 在0.05、0.5和1.0 mg/kg 3个不同加标水平上, 腐霉利和异菌脲的加标回收率为90.6%~102.4%, 精密度为3.72%~6.55%。结论 该方法操作简单、准确, 可应用于番茄中腐霉利和异菌脲的检测。

英文摘要:

      Objective To establish a method for determination of procymidone and iprodione residues in tomato by solid phase extraction-high performance liquid chromatography (SPE-HPLC). Methods After ultrasonic extraction with acetonitrile, the tomato extracts were added sodium chloride to salt out, purified by Florisil column, eluted by the purified solution with n-hexane/acetone (90:10, V:V), blown by nitrogen evaporator until it was almost dry and diluted with methyl alcohol to a constant volume. Then it was separated by a ZORBAX Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) using acetonitrile/water (60:40, V:V) as mobile phase at the flow rate of 1.0 mL/min, and detected by diode array detector (DAD) at 208 nm. Results Procymidone and iprodione had good linear relationships between mass concentration and peak area (r2 >0.999) in the range of 0.05~5.0 mg/L. The detection limits of two pesticides were both 0.025 mg/kg. The recoveries spiked at 3 levels of 0.05, 0.5 and 1.0 mg/kg were 90.6%~102.4% with RSD of 3.72%~6.55%. Conclusion The established method is easy to operate and accurate, which can be applied for the detection of procymidone and iprodione in tomato.Conclusion The method herein is easy to operate and delivers accurate results. Therefore, it can be applied to the diction of procymidone and iprodione in tomato.