液相色谱法测定葡萄酒中柠檬黄、苋菜红、胭脂红、日落黄含量结果的不确定度评定

Uncertainty evaluation for determination of tartrazine, amaranth, carmine, sunset yellow in grape wine by high performance liquid chromatography

中文关键词:  葡萄酒  人工合成着色剂  液相色谱法  不确定度

英文关键词:grape wine  artificial pigment  high performance liquid chromatography  uncertainty

中文摘要:

      目的 评定高效液相色谱法(high performance liquid chromatography, HPLC)测定红酒中人工合成着色剂柠檬黄、苋菜红、胭脂红、日落黄含量的不确定度。方法 依据JJF1059-1999《测量不确定度评定与表示》, 分析人工合成着色剂含量测定过程中的不确定度来源, 通过建立数学模型量化不确定度分量, 计算合成不确定度及扩展不确定度。结果 本研究测得的红酒中人工合成着色剂的含量分别为柠檬黄(52.6±7.2) mg/kg (k=2)、苋菜红(33.6±7.4) mg/kg (k=2)、胭脂红(40.7±5.6) mg/kg (k=2)、日落黄(50.2±6.8) mg/kg (k=2)。测定过程的不确定度来源主要有试样中人工合成着色剂含量、试样质量、试样定容体积、试样稀释倍数等。结论 本方法可反映测量的置信度和准确性, 为日常实际检验工作提供参考。

英文摘要:

      Objective To evaluate the uncertainty of determination of artificial pigments such as tartrazine, amaranth, carmine, sunset yellow in grape wine samples by high performance liquid chromatography (HPLC). Methods According to JJF1059-1999 Chinese regulation on the valuation and expression of uncertainty in measurement, the sources of uncertainty in determination of artificial pigment were analyzed. Furthermore, the combined uncertainties and expanded uncertainties were calculated based on the established mathematic models. Results The content of artificial pigments in grape wine samples in this study were as follows: tartrazine (52.6±7.2) mg/kg (k=2), amaranth (33.6±7.4) mg/kg (k=2), carmine (40.7±5.6) mg/kg (k=2), sunset yellow (50.2±6.8) mg/kg (k=2). And the main sources of uncertainty in the determination were artificial pigments content in the samples, the quality of samples, the constant volume, and dilution multiple of samples. Conclusion This method accurately reflect the confidence and accuracy of the measurement, which can provide reference for the daily actual inspection work.