乳制品中常见食源性致病菌检测技术的研究进展

Research progress of detection technology for familiar foodborne pathogenic bacteria in dairy products

中文关键词:  乳制品  食源性致病菌  检测技术  研究进展

英文关键词:dairy products  foodborne pathogenic bacteria  detection technology  research progress

中文摘要:

      近年来频繁发生的乳与乳制品的质量安全事件引起了人们对乳制品安全的普遍关注, 而食源性致病菌污染是乳制品安全问题的重要隐患之一。乳制品中常见的食源性致病菌有沙门氏菌、蜡样芽孢杆菌、阪崎肠杆菌、李斯特菌和志贺氏菌等。目前食源性致病菌的检测技术主要有国家标准中的培养法检测技术、分子生物学技术和免疫学技术, 分子生物学技术中的PCR检测技术因具有特异性和灵敏度高、简便、快速等优点而得以广泛应用。本文对国家标准中的常规检测技术、分子生物学技术和免疫学技术在乳制品中常见食源性致病菌检测领域的应用进展进行了介绍, 并对其影响因素及存在的问题进行了简要阐述。

英文摘要:

      The frequent quality and safety incidents of milk and dairy products in recent years have caused widespread concern about the safety of dairy products. And foodborne pathogenic bacteria is one of the important hidden trouble of dairy food safety problem. The common foodborne pathogenic bacteria in dairy products are Salmonella, Bacillus cereus, Enterobacter sakazakii, Listeria monocytogenes, Shigella and so on. At present, the detection technologies of foodborne pathogenic bacteria mainly include the methods of culture, molecular biology and immunology, and the PCR detection technology which is a molecular biology technology has a wide range of applications due to its high specificity and sensitivity, simplicity and rapidity. This paper introduced the application progress of conventional national standard detection technologies, molecular biology and immunology technology in dairy products, and their influence factors and existing problems were simply described.