沙门氏菌快速检测板在食品检测中的应用研究

Application of rapid petri-dish in the detection of Salmonella spp. in food

中文关键词:  沙门氏菌  快速检测板  食品检测

英文关键词:Salmonella spp.  rapid petri-dish  food detection

中文摘要:

      目的 研究沙门氏菌快速检测板在食品检测中的应用。方法 以标准菌株为测试样本, 参照GB 4789.4-2010验证沙门氏菌快速检测板的灵敏度、特异性、假阳性率、假阴性率、准确度, 同时利用卡方检验分析阳性率显著性差异。结果 沙门氏菌快速检测板最低检测限能达到100 CFU/mL, 灵敏度为100%, 不存在假阴性, 特异性为92%, 假阳性率为8%, 准确度为95%, 显著性差异卡方值卡Χ2为0.5, 均达到定性方法验证的相关指标。结论 沙门氏菌检测板技术方法具有快速准确、灵敏度高、操作简单的特点, 可以作为一种快速筛查方法广泛运用在食品安全微生物检测领域。

英文摘要:

      Objective To research the application value of rapid petri-dish in the detection of Salmonella spp. in food. Methods The sensitivity, specificity, anti-interference, false positive rate, false negative rate and accuracy of Salmonella spp. petri-dish were studied by using standard strains as specimen and comparison with GB 4789.4-2010. Meanwhile, the significant difference of positive rate was analyzed by the chi-square. Results The minimum detection limit of Salmonella spp. rapid petri-dish could reach 100 CFU/mL. The sensitivity of Salmonella spp. petri-dish was 100%, and the false negative rate was 0. The specificity was 92%, and the false positive rate was 8%. The accuracy rate was 95%, and the chi-square (Χ2) was 0.5. The results showed that all parameters conformed to the requirements of the test. Conclusion It is feasible to detect the Salmonella spp. with the rapid plate as a rapid screening method in the field of food safety, which is sensitivity, specificity, accuracy and simplicity.