实时定量荧光PCR快速鉴定食品中单核细胞增生李斯特氏菌

Rapid detection of Listeria monocytogenes in food by real-time PCR

中文关键词:  单核细胞增生李斯特氏菌  实时定量荧光PCR  食品

英文关键词:Listeria monocytogenes  real-time PCR  food

中文摘要:

      目的 建立实时定量荧光PCR法(real-time PCR)快速鉴定食品中的单核细胞增生李斯特氏菌(Listeria monocytogenes, LM)。方法 选取2016年国家食品风险监测样本134例与模拟灭活LM样本10例, 采用GB/T4789.30-2010与real-time PCR方法同步检测单核细胞增生李斯特菌。结果 共检测食品134份, 包括肉制品、水产品、快餐和即食食品等。共检出8株LM, 检出率为5.97%。以GB/T4789.30-2010为金标准判断, real-time PCR方法检测样本中LM的灵敏度与特异度均达到100%。模拟灭活LM样本real-time PCR方法检出率为100%, 标准法检出率为0%。结论 本方法可以简化实验程序, 减少工作量, 节约检测试剂, 为可能发生的食物中毒尽早提供实验依据。

英文摘要:

      Objective To establish the method for rapid identification of Listeria monocytogenes (LM) in food by real-time PCR. Methods One hundred and thirty-four cases of national food risk monitoring samples in 2016 and 10 cases of simulated inactivated LM samples were simultaneously detected by GB/T4789.30-2010 method and real-time PCR method. Results A total of 134 samples of food including meat products, aquatic products, fast food and instant food etc. were enrolled. Totally 8 strains of LM were detected and the detection rate was 5.97 %. Using GB/T4789.30-2010 as the gold standard, the sensitivity and specificity of real-time PCR were both up to 100%. In 10 samples of simulated inactivated LM, the detection rate of real-time PCR method was 100%, but that of the standard method was 0%. Conclusion The established real-time PCR method can simplify the experiment procedure, reduce the workload and save the reagent, which can provide experimental basis for probable food poisoning as early as possible.