表面等离子共振技术快速检测食品中的 玉米赤霉烯酮毒素

Rapid detection of zearalenone in food by surface plasmon resonance technology

中文关键词:  玉米赤霉烯酮  表面等离子共振  酶联免疫法  真菌毒素

英文关键词:zearalenone  surface plasmon resonance  enzyme linked immunosorbent assay  mycotoxins

中文摘要:

      目的 建立基于表面等离子共振(surface plasmon resonance, SPR)技术快速检测食品中玉米赤霉烯酮毒素(zearalenone, ZEN)的方法。方法 采用表面自组装技术(self-assembled monolayer, SAM)在金膜的表面修饰羧基基团, 将ZEN抗原与牛血清白蛋白(albumin from bovine serum, BSA)偶联物(ZEN-BSA)通过共价键固定在芯片的表面, 采用竞争法检测样品中的玉米赤霉烯酮毒素。结果 该方法的检测限为8.2 ng/mL, ZEN单克隆抗体与呕吐毒素、黄曲霉毒素B1、赭曲霉毒素、伏马毒素等没有交叉反应, 与α-玉米赤霉烯醇和β-玉米赤霉烯醇交叉反应率分别为15.3%和11.5%。结论 本方法具有简便、快速和高灵敏度等优势, 在食品中真菌毒素的快速检测方面具有潜在的应用价值。

英文摘要:

      Objective To establish a method for the rapid detection of zearalenone (ZEN) by surface plasmon resonance (SPR) technology. Methods The surface of the gold film was modified with carboxy group by self-assembled monolayer (SAM). The ZEN-BSA conjugate was immobilized on SPR chip through covalent bond, and the competition method was used to detect ZEN in food. Results The detection limit of this method was 8.2 ng/mL. The ZEN monoclonal antibody did not show cross reaction with deoxynivalenol, aflatoxin B1, ochratoxin and fumonisin, and the cross reaction rates with α-zearalenol and β-zearalenol were respectively 15.3% and 11.5%. Conclusion The established method is simple, rapid, highly sensitive, which has the potential application value in the rapid detection of mycotoxins in food.