中压制备色谱在天然产物分离纯化中的应用

Applications of medium pressure liquid chromatography in separation and purification of natural products

中文关键词:  中压制备色谱  高速逆流色谱  天然产物  分离纯化

英文关键词:medium pressure liquid chromatography  high-speed countercurrent chromatography  natural product  separation and purification

中文摘要:

      中压制备色谱(medium pressure liquid chromatography, MPLC)是相对于常压色谱和高效液相色谱(high performance liquid chromatography, HPLC)而言, 其压力介于0~200 psi之间, 主要应用于天然产物化学、生物化学、药物化学、有机合成以及生命科学等领域产品的分离与纯化。MPLC一般由计量泵、检测器、收集器和工作站组成。MPLC最大特点是分离速度快、效率高, 可短时间内制备毫克到数十甚至百克的样品, 可应用于正相填料和反相填料。与常压柱色谱相比, 它具有分辨率高、分离速度快的优势和制备量大、时间短的特点。近年来, MPLC已逐渐发展为一种备受欢迎的分离和纯化方法。本文主要介绍了MPLC的特点、与高速逆流色谱的比较以及反相MPLC在分离纯化中的应用等。

英文摘要:

      Medium pressure liquid chromatography (MPLC) is relative to the atmospheric pressure chromatography and high performance liquid chromatography (HPLC), and its pressure is between 0~200 psi, which is mainly applied for the separation and purification in the fields of natural product chemistry, biochemistry, pharmaceutical chemistry, organic synthesis and life science products, etc. MPLC generally consists of metering pump, detector, collector and workstation. The biggest characteristics of MPLC were high separation speed, high efficiency, and preparation of milligrams to dozens or even up to hundreds of grams of samples within a short time. It can be used both in the positive phase filler and the reverse packing. Compared with the normal pressure column chromatography, MPLC has the advantages of high resolution, fast separation and the characteristics of large amount of preparation and short time. In recent years, MPLC has gradually developed into a popular method for separation and purification. This paper mainly introduced the characteristics of MPLC, comparison with high-speed countercurrent chromatography and the application of reversed-phase MPLC in the separation and purification and so on.