高效液相色谱法测定糕点、糖果、月饼中的 2-(4-甲氧基苯氧基)丙酸钠

Determination of sodium 2-(4-methyoxy-phenoxy)propanoate in pastry, candy and mooncake by high performance liquid chromatography

中文关键词:  高效液相色谱法  2-(4-甲氧基苯氧基)丙酸钠  糕点  糖果  月饼

英文关键词:high performance liquid chromatography  2-(4-methyoxy-phenoxy)propanoate  pastry  candy  mooncake

中文摘要:

      目的 采用高效液相色谱法测定糕点、糖果和月饼中的2-(4-甲氧基苯氧基)丙酸钠的含量。方法 样品经超声提取后, 采用Diamonsil C18色谱柱分离, 以甲醇:乙腈:水(50:10:40, V:V:V, pH=3)为流动相, 在柱温为25 ℃、流速为0.8 mL/min的条件下进行洗脱, 采用二极管阵列检测器在波长224 nm下对食品中2-(4-甲氧基苯氧基)丙酸钠进行检测, 以外标法定量。结果 在0.50~200 mg/L浓度范围内, 2-(4-甲氧基苯氧基)丙酸钠具有良好的线性关系; 该方法定量限为0.01 g/kg, 检出限为0.003 g/kg; 加标浓度水平为1.000 mg/L时, 糕点、糖果和月饼平均加标回收率分别为92.8%~95.8%、95.3%~96.8%和94.0%~98.5%, 重复性试验的相对标准偏差分别为3.5%~4.5%、2.8%~3.8%和3.0%~4.8%(n=10)。结论 该方法具有较高的准确性和良好的重现性, 适合食品中2-(4-甲氧基苯氧基)丙酸钠的测定。

英文摘要:

      Objective To establish a method for determination of sodium 2-(4-methyoxy-phenoxy)propanoate in pastry, candy and mooncake by high performance liquid chromatography. Methods The sample was extracted with ultrasound, separated by Diamonsil C18 column with methanol:acetonitrile:water (50:10:40, V:V:V, pH=3) as mobile phase at the flow rate of 0.8 mL/min and the column temperature of 25 ℃. And then 2-(4-methoxy phenoxy)sodium propionate in food was detected by diode array detector at the wavelength of 224 nm, and quantified by external standard method. Results 2-(4-methoxy phenoxy)propionate had a good linear relationship in the concentration range of 0.50~200 mg/L. The limit of quantification was 0.01 g/kg and the limit of detection was 0.003 g/kg. The recoveries of pastries, candy and mooncakes and were 92.8%~95.8%, 95.3%~96.8% and 94.0%~98.5%, respectively, and the relative standard deviations of repeatability test were 3.5%~4.5%, 2.8%~3.8% and 3.0%~4.8% (n=10), respectively. Conclusion The method is accurate and precise, which is suitable for the determination of 2-(4-methyoxy-phenoxy)propanoate in food.