高效液相色谱法测定火锅汤底中的咖啡因

Determination of caffeine in hot pot soup by high performance liquid chromatography

中文关键词:  高效液相色谱法  火锅汤底  咖啡因

英文关键词:high performance liquid chromatography  hot pot soup  caffeine

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定火锅汤底中咖啡因的含量。方法 样品在碱性条件下经三氯甲烷提取, 采用Phenomenex C18色谱柱分离, 以甲醇:水:乙酸=10:89:1(V:V:V)为流动相等度洗脱, 流速为1.0 mL/min, 柱温为40 ℃。采用二极管阵列检测器在286 nm波长下进行检测, 以外标法定量。结果 咖啡因在1~100 mg/kg浓度范围内具有良好的线性关系, 相关系数为0.9990, 检出限为2.00×10-3 mg/kg, 在20.00和50.00 mg/kg 2个添加水平上的加标回收率为87.0%~93.6%, 相对标准偏差为3.01%~5.91%。结论 该方法操作简单, 灵敏度高, 重复性好, 可适用于火锅汤底中咖啡因的测定。

英文摘要:

      Objective To establish a method for determination of caffeine in hot pot soup by high performance liquid chromatography (HPLC). Methods Samples were extracted with trichloromethane under the alkaline condition, separated by Phenomenex C18 column with methyl alcohol:water:acetic acid (10:89:1, V:V:V) as mobile phase for isocratic elution at a flow rate of 1.0 mL/min at 40 ℃, and then detected by diode array detector at 286 nm. The quantification was performed by the external standard method. Results Caffeine had a good linear relationship in the range of 1~100 mg/kg with correlation coefficients(r2) of 0.9990. The detection limit was 2.00×10-3 mg/kg. The average recoveries for caffeine spiked at 2 levels of 20.00 and 50.00 mg/kg were in the range of 87.0%~93.6% with relative standard deviations (RSDs) of 3.01%~5.91%. Conclusion With the advantages of simplicity, sensitivity and repeatability, the established method can meet the requirements for determination of caffeine in hot pot soup.