山西老陈醋中总黄酮测定的不确定度评价

Uncertainty evaluation of the determination of total flavone in Shanxi mature vinegar

中文关键词:  山西老陈醋  总黄酮  不确定度

英文关键词:Shanxi mature vinegar  total flavone  uncertainty

中文摘要:

      目的 研究山西老陈醋中总黄酮含量测定方法的不确定度。方法 以芦丁作为对照品, 对GB/T 19777-2013法中山西老陈醋总黄酮含量测定的不确定度进行评定。结果 按照此标准测定总黄酮含量为283.4 mg/100 g的山西老陈醋, 取包含因子k=2(包含概率为95%), 扩展不确定度为11.3 mg/100 g, 其计算结果可表示为(283.4±11.3) mg/100 g。测定结果的不确定度主要来源于绘制标准曲线的过程中的移取、定容过程。结论 在实际检测工作中, 要确保绘制标准曲线过程中容量瓶、移液管的容量准确和移取、定容操作的规范性。

英文摘要:

      Objective To investigate the uncertainty of the determination of total flavone in Shanxi mature vinegar. Methods According to GB/T 19777-2013, uncertainty of the determination of total flavone in Shanxi mature vinegar was evaluated, using rutinum as a reference material. Results The total flavone in Shanxi mature vinegar was 283.4 mg/100 g, and the expanded uncertainty was 11.3 mg/100 g with k=2 (the coverage probability was 95%), then the value could be expressed as (283.4±11.3) mg/100 g. The main source of the measurement uncertainty was the preparation of standard curve in the determination of total flavone in Shanxi mature vinegar. Conclusion In the actual detection work, we must ensure the accurate capacity of volumetric flask and pipette and normative operation in the process of drawing standard curve.