牛奶中主要过敏原αs1-酪蛋白的生物信息学分析

Bioinformatics analysis for αs1-casein of main allergens in milk

中文关键词:  过敏原  αs1-酪蛋白  生物信息学  牛奶

英文关键词:allergen  αs1-casein  bioinformatics  milk

中文摘要:

      目的 用生物信息学方法分析预测牛奶中主要过敏原αs1-酪蛋白的结构与功能。方法 从Genbank搜索αs1-酪蛋白的氨基酸序列, 采用多种生物信息学分析手段对该序列进行分析预测, 包括信号肽、疏水性、跨膜区、活性位点、B细胞表位、二级结构和三级结构。结果 αs1-酪蛋白的1~16位是信号肽, 无跨膜区, 有3个糖基化位点、5个酪蛋白激酶磷酸化位点、3个蛋白激酶C磷酸化位点。IEDB数据库预测αs1-酪蛋白的抗原区分别为氨基酸的16~29、45~50、52~54、56~70、74~75、77~86、94~104、142~152、173~179和185~210, 同时预测构建了αs1-酪蛋白的二级结构和三级结构。结论 预测得到的αs1-酪蛋白结构和功能信息, 可为牛奶过敏原酪蛋白的致敏机制研究及检测方法建立提供信息基础。

英文摘要:

      Objective To analyze and predict the structures and functions of main allergen αs1-casein in milk by bioinformatics methods. Methods The amino acid sequence of αs1-casein was searched in Genbank. Its signal peptides, hydrophobicities, transmembrane domains, active site, B-cell epitopes, the secondary structures and tertiary structure were predicted and analyzed by bioinformatics methods. Results The predicted signal peptide was in the 1~16 amino acid secquence. It had no transmembrane region, 3 glycosylation sites, 5 casein kinase phosphorylation sites and 3 protein kinase C phosphorylation sites in the protein. After prediction by IEDB, the antigen areas of αs1-casein were in the amino acid sites of 16~29, 45~50, 52~54, 56~70, 74~75, 77~86, 94~104, 142~152, 173~179 and 185~210, respectively. Meanwhile, the secondary structure and tertiary structure were predicted and built. Conclusion The predicated structures and function information of αs1-casein can provide information base for the sensitization mechanism research and detection of casein allergens in milk.