高效液相色谱法同时测定酱腌菜中15种 食品添加剂的含量

Simultaneous determination of 15 kinds of food additives in pickles by high performance liquid chromatography

中文关键词:  高效液相色谱法  酱腌菜  食品添加剂  同时检测

英文关键词:高效液相色谱法  酱腌菜  食品添加剂  同时检测

中文摘要:

      目的 建立同时测定酱腌菜中15种食品添加剂的高效液相色谱法。方法 样品经过提取净化后, 在优化的梯度洗脱条件下分离, 二极管阵列检测器进行检测。结果 15种食品添加剂在给定的浓度范围内各添加剂的浓度与峰面积具有良好的线性关系, 相关系数(r)在0.9991~0.9998之间。该方法检出限为3.0~6.5 mg/kg, 加标回收率为86.4%~103.4 %, 相对标准偏差为2.48%~8.10 %。结论 该方法回收率高、精密度高、准确度高、重现性好, 是测定酱腌菜中15种添加剂的有效方法。

英文摘要:

      Objective To establish a method for the simultaneous determination of 15 kinds of food additives in pickles by high performance liquid chromatography (HPLC). Methods Samples were extracted and purified, then separated under the optimized gradient conditions and finally detected by HPLC with diode array detector. Results All the compounds exhibited good liner relationships with the correlation coefficients (r) of 0.9991~0.9998. The detection limits were 3.0~6.5 mg/kg, and average recoveries were 86.4%~103.4% with RSDs of 2.48%～8.10%. Conclusion The method is suitable for the determination of 15 kinds additives in pickles which has a high recovery rate, precision, accuracy and a good reproducibility.