分子生物学技术在食源性致病菌检测中的 研究进展

Research progress of molecular biology techniques on detecting foodborne pathogens

中文关键词:  分子生物学技术  食源性致病菌  检测方法  食品安全

英文关键词:molecular biological technology  foodborne pathogens  detection method  food safety

中文摘要:

      食源性致病菌是导致食源性疾病的重要爆发根源之一。分子生物学检测技术因其敏感性强、特异性高、快速简便、省时省力等优点, 在食源性致病菌的鉴定与检测中扮演着重要角色。本文系统阐述了利用分子生物学技术检测食源性致病菌的方法, 包括多重PCR、实时荧光定量PCR、环介导等温扩增技术、基因芯片、液相芯片和基因探针技术等, 分析了目前国内外学者对于这些技术由单一检测向多重检测的突破, 及实现快速、高通量检测食源性致病菌的应用研究成果, 总结了各种检测技术的优缺点, 旨在为未来更好地发展快速、高通量检测食源性致病菌的技术方法提供参考。

英文摘要:

      Foodborne diseases are mainly caused by foodborne pathogens. Molecular biological detection technology which possesses the advantages of high sensitivity, high specificity, rapid and easy, and time-saving, and so on plays an important role in identification and detection of food-borne pathogens. This paper elaborated systematically the molecular biology technique adopted in detection of food-borne pathogens, including traditional PCR, multiple PCR, real-time fluorescent quantitative PCR, loop-mediated isothermal amplification technology, gene chips, liquid phase chips, and gene probes, and analyzed the breakthrough of single to multiple detection and research results of rapid and high-throughput detection of food borne pathogens from studies at home and abroad. Finally, the paper summarized the advantages and disadvantages of various detection techniques for the purpose that providing a reference for the better development of rapid, high-throughput detection food-borne pathogens in the future.