渔用麻醉剂MS-222的电子轰击电离质谱 裂解规律研究

Fragmentation pattern of fish anesthetic MS-222 by electron ionization mass spectrometry

中文关键词:  麻醉剂  MS-222  电子轰击电离  裂解规律

英文关键词:anesthetic  MS-222  electron ionization  cleavage pathways

中文摘要:

      目的 建立气相色谱-串联质谱(GC-MS/MS)法研究渔用麻醉剂MS-222电子轰击电离的裂解途径的分析方法。方法 针对MS-222, 采用电荷和游离基理论, 解释主要碎片离子的裂解机制。结果 MS-222经电子轰击电离产生特征碎片离子m/z 150、137、120和93; 分子离子以饱和氧和不饱和氧为电荷/游离基中心引发α断裂和酯基上伴随γ重排的β断裂, 碎片离子进一步发生简单α裂、消除反应等反应, 产生相应的特征碎片离子。结论 电子轰击电离结合GC-MS/MS可用于鱼类麻醉剂MS-222的质谱断裂机制研究, 方法简便, 可推广到其他鱼类麻醉剂的研究中去。

英文摘要:

      Objective To establish a method for the investigation of the fragmentation pathways of anesthetic MS-222 under electron ionization (EI) condition by gas chromatography tandem mass spectrometry (GC-MS/MS)．Methods The fragmentation pathways of MS-222 were gotten and interpreted using radical-site and charge-site initiation theory．Results The main fragment ions of MS-222 were m/z 150, 137, 120 and 93. The reaction of molecular ions initiated at the radical-site and charge-site of unsaturated oxygen and saturated oxygen. The molecular ions produced different fragment ions from the α-cleavage reaction and inductive-cleavage reaction with hydrogen rearrangement. These fragment ions continued to produce other ions from α-cleavage, elimination reaction and other reactions. Conclusion EI combined with GC-MS/MS can be used for the investigation of the fragmentation mechanism of fish anesthetic MS-222. This method is simple and convenient, and can be extended to the study of other fish anesthetics.