不同症状小麦赤霉病粒中脱氧雪腐镰刀菌烯醇的含量分析

Content analysis of deoxynivalenol in Gibberella damaged grains of wheat with different disease symptoms

中文关键词:  小麦赤霉病粒  高效液相色谱法  脱氧雪腐镰刀菌烯醇

英文关键词:Gibberella damaged grains of wheat  high performance liquid chromatography  deoxynivalenol

中文摘要:

      目的 测定不同症状的小麦赤霉病粒中脱氧雪腐镰刀菌烯醇(DON)的含量。方法 采用免疫亲和层析净化高效液相色谱法测定健康、皱缩、皱缩发黑和皱缩发红的小麦籽粒中脱氧雪腐镰刀菌烯醇含量。结果 健康麦粒中DON的含量为未检出, 皱缩红色和皱缩发黑的麦粒中DON的含量均接近我国食品安全国家标准规定的食品中真菌毒素限量1000 μg/kg; 皱缩麦粒中DON的含量约为皱缩红色麦粒中的一半, 极显著低于皱缩红色和皱缩发黑的麦粒(P＜0.01)。结论 本方法能为粮食收购时判断DON毒素的含量提供理论依据。

英文摘要:

      Objective To detect the content of deoxynivalenol (DON) in Gibberella damaged grains of wheat with different disease symptoms. Methods The content of DON in grains of wheat with healthy, shrinkage, shrinkage and black, shrinkage and red symptoms were detected by immune affinity chromatography purification with high performance liquid chromatography, respectively. Results The content of DON in healthy grains of wheat was not detected, and the content of DON in grains of wheat with shrinkage and black, shrinkage and red symptoms were close to 1000 μg/kg which was the permitted level of national food safety standards in food mycotoxins standard. The content of DON in grains of wheat with shrinkage symptom was about half of that of red wheat, and was significantly lower than that of shrinkage and red wheat and shrinkage and black wheat (P<0.01). Conclusion It can provide theoretical basis for grain procurement agencies to determine the content of DON.