高效液相色谱法测定中国劲酒中桂皮醛的含量

Determination of cinnamaldehyde in Chinese Jing Wine by high performance liquid chromatography

中文关键词:  中国劲酒  桂皮醛  高效液相色谱法

英文关键词:Chinese Jing Wine  cinnamaldehyde  high performance liquid chromatography

中文摘要:

      目的 建立采用高效液相色谱法(high performance liquid chromatography, HPLC)测定中国劲酒中桂皮醛含量的方法。方法 样品以10%甲醇为提取剂, 超声提取30 min, 以乙腈-水(35:65, V:V)为流动相, 流速为1.0 mL/min, 采用Shimadzu VP-ODS色谱柱(250 mm×4.6 mm, 5 μm)分离, 在290 nm检测波长下经HPLC检测, 同时对流动相比例和提取剂等条件进行优化。结果 桂皮醛浓度在0.0216~0.216 μg/mL范围内呈良好的线性关系, 相关系数为0.99998, 平均回收率为98.1%, 相对标准偏差为1.63%, 方法检出限为0.1 μg/mL。结论 本方法操作简便﹑结果准确且重现性好, 可用于中国劲酒中桂皮醛含量的测定。

英文摘要:

      Objective To establish a method for the determination of cinnamaldehyde in Chinese Jing Wine by high performance liquid chromatography (HPLC). Methods Samples were extracted with 10% methanol by ultrasonic for 30 min, and separated by a Shimadzu VP-ODS column (250 mm×4.6 mm, 5 μm) with acetonitrile-water (35:65, V:V) as mobile phase at the flow rate of 1.0 mL/min. Then the samples were detected by HPLC at the detection wavelength of 290 nm. Meanwhile, the mobile phase ratio and extractant were optimized. Results The cinnamaldehyde had a good linearity relationship at the range of 0.0216~0.216 μg/mL, and the correlation coefficient was 0.99998. The average recovery was 98.1%, the relative standard deviation was 1.63%, and the detection limit was 0.1 μg/mL. Conclusion The method is rapid, accurate and reproducible, which can be used for the determination of cinnamaldehyde in Chinese Jing Wine.