气相色谱法分析不同产地辣木叶中植物甾醇的含量

Content determination of phytosterol in Moringa oleifera leaves from different areas by gas chromatography

中文关键词:  气相色谱  辣木叶  植物甾醇

英文关键词:gas chromatography  Moringa oleifera leaves  phytosterol

中文摘要:

      目的 研究西双版纳、韶关、安哥拉、印度和云南辣木叶中菜油甾醇、β谷甾醇和豆甾醇的含量差异。方法 样品经50%氢氧化钾皂化后, 用乙醚:正己烷(1:1,V:V)萃取游离甾醇并将溶剂蒸干, 用N,N-二甲基甲酰胺:六甲基二硅氨烷:三甲基氯硅烷(8:2:1,V:V:V)将游离甾醇硅烷化, 然后进行气相色谱分析, 并分析各地辣木叶中菜油甾醇、豆甾醇和β谷甾醇含量。结果 β谷甾醇在韶关辣木叶中含量为20 mg/100 g左右, 在西双版纳辣木叶中含量为40 mg/100 g,在安哥拉、印度和云南辣木叶中含量为30 mg/100 g左右; 菜油甾醇在韶关辣木叶中含量为5~7 mg/100 g, 在印度辣木叶中含量为8 mg/100 g,在西双版纳、安哥拉和云南辣木叶中均大于10 mg/100 g;豆甾醇在各个辣木叶中含量为2~12 mg/100 g。结论 西双版纳、云南、安哥拉、印度辣木叶中菜油甾醇、β谷甾醇含量明显高于韶关辣木叶, 豆甾醇含量没有明显的地域差异。

英文摘要:

      Objective To study the content difference of campesterol, β-sitosterin, and stigmasterol in Moringa oleifera leaves from Xishuangbanna, Shaoguan, Angola, India and Yunnan. Methods After saponification by 50% potassium hydroxide, the free phytosterol of samples were extracted by diethyl ether:hexane(1:1,V:V), and the solvent were evaporated to dryness before silanization using N,N-Dimethylformamide(DMF), hexamethyldisilazane (HMDS) and trimethyl-chlorosilane (TMCS)(8:2:1,V:V:V). The content of campesterol, stigmasterol and β-sitosterin in Moringa oleifera leaves from various areas were analyzed by gas chromatography (GC). Results The content of β-sitosterin were around 20 mg/100 g in Moringa oleifera leaves from Shaoguan, 40 mg/100 g in that from Xishuangbanna and 30 mg/100 g in those from Angola, India and Yunnan. The content of campesterol were around 5~7 mg/100 g in Moringa oleifera leaves from Shaoguan, 8 mg/100 g in that from India and larger than 10 mg/100 g in those from Xishuangbanna, Angola and Yunnan. The content of stigmasterol were 2~12 mg/100 g in Moringa oleifera leaves from different areas. Conclusion The content of campesterol and β-sitosterin of Moringa oleifera leaves from Xishuangbanna, Yunnan, Angola and India are remarkably higher than those from Shaoguan. The content of stigmasterol has no obvious regional differences.