超声辅助提取螺旋藻粉中叶绿素a的工艺研究

Research on ultrasonic assisted extraction of chlorophyll a from Spirulina powder

中文关键词:  螺旋藻粉  叶绿素a  超声辅助提取法

英文关键词:Spirulina powder  chlorophyll a  ultrasonic assisted extraction

中文摘要:

      目的 对超声辅助提取螺旋藻粉中的叶绿素a工艺进行优化。方法 设计4因素3水平进行正交实验, 即以提取时间、提取溶剂浓度、料液比和提取温度为实验因素, 每个实验因素设计3个水平。通过分析极差和均值, 筛选出最优的提取工艺条件。结果 最佳工艺条件如下: 超声时间50 min, 乙醇浓度80 %, 超声温度35 ℃, 料液比35:50(m:V)可以达到较理想的提取效果, 其中乙醇浓度和超声时间是影响提取叶绿素a的主要因素, 其次分别为超声温度和料液比。结论 本研究筛选出的超声辅助提取条件准确、重现性好, 可适用于螺旋藻粉中叶绿素a含量的测定。

英文摘要:

      Objective To optimize the ultrasonic assisted extraction of chlorophyll a from Spirulina powder. Methods The orthogonal experimentof 4factors and3 levels was designed. The extraction time, extraction solvent concentration , solid-liquid ratio and extraction temperature were regraded as the 4 experimental factors with each experimental factor designedfor 3levels. The optimum extraction conditions were carried out by analyzing the means and extreme values. Results The optimum process conditions were as follows: the ultrasonic time was 50min, ethanol concentration was 80%, ultrasonic temperature was 35 ℃and solid-liquid ratio was 35: 50 (m:V), which could achieve good extraction effect. The concentration of ethanol and time wereknown as the main influencing factors fortheextraction of chlorophyll a, followed by ultrasound temperature and liquor ratio. Conclusion The ultrasound-assisted extraction conditionscreened in this study is accurate, reproducible, which is suitable for the determination of chlorophyll a in Spirulina powder.