动植物油脂中丙二醛测定方法的优化

Amendment of method for determination of propyldialdehyde in animal and vegetable oils

中文关键词:  动物油脂  植物油脂  丙二醛  测定方法  优化

英文关键词:animal oil  vegetable oils  propyldialdehyde  determination method  optimization

中文摘要:

      目的 对GB/T 5009.181-2003和GB/T 8937-2006中食用动植物油脂中丙二醛的测定方法进行优化。方法 样品采用三氯乙酸混合溶液经50 ℃恒温水浴震荡提取, 与硫代巴比妥酸(thiobarbituric acid, TBA)作用生成粉红色化合物, 在532 nm处采用分光光度计法测定。结果 丙二醛在0~2.5 mg/L范围内线性关系良好, r=0.9995, 方法检出限为0.1 mg/kg, 回收率在88.0%~100.0%之间, 日内精密度和日间精密度小于6.09 %。结论 该方法扩大了检测范围, 操作简便准确, 灵敏度高, 可适用于动植物油脂中丙二醛的测定。

英文摘要:

      Objective To optimize the method of GB/T 5009.181-2003 and GB/T 8937-2006 fordetermination of propyldialdehyde in animal and vegetable oils. Methods Samples were extracted with trichloroacetic acid solvent at 50 ℃ by constant temperature water bath oscillators, the filtered solution was derivated by thiobarbituric acid for spectrophotometric test at a wavelength of 532 nm. Results Propyldialdehyde had a good linearity in the range of 0~2.5 mg/L with the correlation coefficient of 0.9995. The limit of detection was 0.1 mg/kg, and the recovery was in the range of 88.0%~100.0% with inter-day precision and within-day precision less than 6.09%. Conclusion The improved method expands the scope of tests, which is more simple, rapid, accurate, and suitable for the determination of propyldialdehyde in animal and vegetable oils.