高效液相色谱法同时测定复方中药制剂中的 芍药苷、甘草酸和丹参酮ⅡA的含量

Simultaneous determination of paeoniflorin, glycyrrhizic acid and tanshinone ⅡA in compound traditional Chinese medicine preparation by high performance liquid chromatography

中文关键词:  芍药苷  甘草酸  丹参酮ⅡA  高效液相色谱法

英文关键词:paeoniflorin  glycyrrhizic acid  tanshinone ⅡA  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定复方中药制剂中芍药苷、甘草酸和丹参酮ⅡA的含量。方法 样品经50%乙醇超声提取, 经Alltima-C18柱分离, 以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱, 采用可变波长检测器(variable wavelength detector, VWD) 在250 nm波长处进行检测。结果 芍药苷、甘草酸和丹参酮ⅡA能实现较好的分离, 在各自线性范围内线性关系良好; 平均回收率为97.5~102.4%, RSD＜2.0%。结论 该方法操作快速简便、精密度和重复性好, 可适用于复方中药制剂中芍药苷、甘草酸和丹参酮ⅡA的同时测定。

英文摘要:

      Objective To establish a method for simultaneous determination of paeoniflorin, glycyrrhizic acid and tanshinone ⅡA in compound traditional Chinese medicine preparation by high performance liquid chromatography (HPLC). Methods Samples were ultrasonically extracted with 50% ethanol, separated by Alltima-C18 column with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution, and then detected by variable wavelength detector (VWD) at the wavelength of 250 nm. Results Paeoniflorin, glycyrrhizic acid and tanshinone ⅡA could be completely separated, and had good linear relationships in their linear ranges. The average recoveries were 97.5%~102.4% with RSDs＜2.0%. Conclusion The method is rapid, simple, accurate and repeatable, which can be used for the simultaneous determination of paeoniflorin, glycyrrhizic acid and tanshinone ⅡA in compound traditional Chinese medicine preparation.