马来西亚与印度尼西亚产燕窝中唾液酸含量的 差异分析

Variance analysis of sialic acid content in Malaysia and Indonesia bird’s nest production

中文关键词:  唾液酸  含量差异  燕窝  马来西亚  印度尼西亚

英文关键词:sialic acid  content difference  bird’s nest  Malaysia  Indonesia

中文摘要:

      目的 测定马来西亚和印度尼西亚产燕窝中的唾液酸含量, 探究其是否与产地存在相关性。方法 样品经乙酸加热水解释放出唾液酸, 水解液经Agilent 300-SCX色谱柱分离, 采用乙腈-0.1%磷酸水溶液(90:10, V:V)作为流动相, 采用二极管阵列检测器在205 nm波长下进行高效液相色谱检测。结果 对30份燕窝样本中的唾液酸含量进行分析, 马来西亚产地燕窝中唾液酸含量范围为7.00~11.9 g/100 g, 平均含量为9.11 g/100 g, 印尼产地燕窝唾液酸含量范围为7.89~12.8 g/100 g, 平均含量为10.5 g/100 g。通过统计学t检验分析, 印尼产燕窝唾液酸含量略高于马来西亚产的燕窝, 但不存在显著性差异(P>0.05)。结论 燕窝唾液酸不会对不同产国的燕窝品质带来显著影响。

英文摘要:

      Objective To investigate the sialic acid content in bird's nests from Malaysia and Indonesia, so as to explore its correlation with origin. Method Samples were hydrolyzed in heat acetic acid solution for releasing of sialic acid, the hydrolysis liquid was separated by Agilent 300-SCX column using acetonitrile-0.1% phosphoric acid water solution (90:10, V:V) as the mobile phase, and then detected by high performance liquid chromatography (HPLC) equipped with diode array detection (DAD) at the wavelength of 205 nm. Results The sialic acid content of 30 bird's nest samples were analyzed, sialic acid content of bird's nest samples from Malaysia and Indonesia were in the ranges of 7.00~11.9 g/100 g and 7.89~12.8 g/100 g, and the average content were 9.11 g/100 g and 10.5 g/100 g, respectively. Through statistical t test method analysis, sialic acid contents of bird's nest samples from Indonesia were slightly higher than that of Malaysia, but there was no significant difference (P>0.05). Conclusions The sialic acid content does not significantly influence on the quality of bird's nest from different countries.