高效液相色谱法同时测定豆浆中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素 5种香料

Simultaneous determination of maltol, ethyl maltol, vanillin, methyl vanillin and ethyl vanillin in soya bean milk by high performance liquid chromatography

中文关键词:  麦芽酚  乙基麦芽酚  香兰素  甲基香兰素  乙基香兰素  豆浆  高效液相色谱法

英文关键词:maltol  ethyl maltol  vanillin  methyl vanillin  ethyl vanillin  soya bean milk  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定豆浆中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素5种香料的含量。方法 样品经沉淀蛋白后用水稀释, 采用Thermo Hypersil-Gold C18色谱柱分离, 以0.1%甲酸溶液-甲醇为流动相进行梯度洗脱, 在273 nm波长下进行高效液相色谱检测。结果 在1~100 μg/g浓度线性范围内, 麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素呈现良好的线性关系, 相关系数大于0.999, 在2、5、10和20 μg/g 4个添加水平下, 加标回收率为63.39%~88.73%, RSD为0.9%~4.5% (n=6), 检出限在0.04~0.18 μg/g之间。结论 该方法灵敏度高、重复性好, 可应用于实际样品的测定。

英文摘要:

      Objective To establish a method for simultaneous determination of maltol, ethyl maltol, vanillin, methyl vanillin and ethyl vanillin in soya bean milk by high performance liquid chromatography (HPLC). Methods After protein deposition, the samples were diluted by water, separated by Thermo Hypersil-Gold C18 column with the mobile phase of 0.1% formic acid-methanol for gradient elution, and detected at the wavelength of 273 nm by HPLC. Results Maltol, ethyl maltol, vanillin, methyl vanillin and ethyl vanillin had good linear relationships in the range of 1~100 μg/g with correlation coefficients higher than 0.999. The individual average recoveries spiked at 4 levels of 2, 5, 10 and 20 μg/g were ranged from 63.39% to 88.73%, while the relative standard deviations (RSD) were 0.9%~4.5% (n=6). The limits of detection were 0.04~0.18 μg/g. Conclusion The established method has high sensitivity and good repeatability, which can be applied for testing actual samples.