超高效液相色谱-三重串联四级杆质谱法测定 乌梅中19种深色染色色素

Detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry

中文关键词:  乌梅  深色色素  染色  超高效液相色谱-三重串联四级杆质谱法

英文关键词:Mumu Fructus  dark pigment  dye  ultra high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 采用超高效液相色谱-三重串联四级杆质谱法建立乌梅中蓝色、绿色、黑色等多种色素检测方法。方法 药材经甲醇-0.1%甲酸水(V:V=3:2)超声提取3次, 提取液合并, 分别经弱阴离子WAX固相萃取柱和弱阳离子WCX固相萃取柱净化得到水溶性酸性色素待测溶液和水溶性碱性色素待测溶液, 最后采用超高效液相色谱-三重串联四级杆质谱仪分析测定。结果 19种色素的加标回收率62.3%～128.2%, 方法检测限为0.5～345.5 μg/kg。将本方法用于实际样品分析, 检出部分乌梅有非法染色。结论 此方法灵敏、快速, 可用于测定乌梅非法染色。

英文摘要:

      Objective To establish a method for detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Methods Samples were extracted with methanol-0.1% formic acid solution (V:V=3:2) by ultrasonic 3 times, cleaned up with WAX and WCX columns to obtain acidic and alkaline pigments tested solution, respectively. Finally, the analytes were detected by UHPLC-MS/MS. Results Recoveries of 19 kinds of dark pigments in Mumu Fructus were 62.3%～128.2%, and the limits of detection were 0.5～345.5 μg/kg. This method could be applied to sample analysis, and the results found some partial illegally added pigments in Mumu Fructus. Conclusion The established method is fast and sensitive, which can meet the determination requirements of dark pigments illegally added in Mumu Fructus.