反相高效液相色谱法测定桃金娘果酒中5种 酚类物质的含量

Determination of 5 kinds of polyphenols in myrtle fruit wine by reversed phase high performance liquid chromatography

中文关键词:  果酒  酚类物质  原儿茶酸  儿茶素  咖啡酸  阿魏酸  白藜芦醇  反相高效液相色谱法

英文关键词:fruit wine  polyphenols  protocatechuic acid  resveratrol  caffeic acid  ferulic acid  catechins  reversed phase high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定桃金娘果酒中原儿茶酸、咖啡酸、阿魏酸、白藜芦醇和儿茶素5种酚类物质含量的检测方法。方法 以乙酸乙酯为萃取溶剂,对酚类物质进行萃取,采用phenemenex C18(4.6mm×250mm,5μm)反相色谱柱,紫外检测器,检测波长为280nm,流动相为 0.4%甲酸-甲醇,梯度洗脱,流速为1.0mL/min,柱温为30℃。结果 在测试范围内,5种酚类物质的浓度与相应的峰面积呈线性关系,相关系数(r)均大于0.999,加标回收率范围在99.15%-102.27%,相对标准偏差小于3.0%。结论 该方法操作简单,灵敏度高,可用于桃金娘果酒中5种酚类物质的含量测定。

英文摘要:

      Objective To establish a method for the determination of 5 kinds of polyphenols including protocatechuic acid, resveratrol, caffeic acid, ferulic acid and catechins in fruit wine of myrtle by reversed phase high performance liquid chromatography (RP-HPLC). Methods Polyphenols were extracted with ethyl acetate, separated by the analytical column of Phenemenex C18 (4.6 mm×250 mm, 5 μm) with the mobile phase of methanol and 0.04% methanol acid water for gradient elution at the flow rate of 1.0 mL/min, and the column temperature was 30 ℃, the wavelength of UV detector was 280 nm. Results Protocatechuic acid, resveratrol, caffeic acid, ferulic acid and catechins showed good linear relationships (r≥0.9993) within the ranges of 0.012~0.20 mg/mL, the recoveries ranged from 98.9% to 100.9% with RSDs<2.1%. Conclusion The method is sensitive and accurate, which can be applied for determination of polyphenols in myrtle fruit wine.