马尾松花粉提取物对小鼠非酒精性脂肪肝的预防作用

Preventive effect of Pinus massoniana pollen extract on nonalcoholic fatty liver in mice

中文关键词:  马尾松花粉提取物  非酒精性脂肪肝  预防作用

英文关键词:masson pine pollen extract  non-alcoholic fatty liver  preventive effect

中文摘要:

      目的 研究马尾松花粉提取物对小鼠非酒精性脂肪肝的预防作用, 并探讨潜在的作用机制。方法 将60只小鼠随机分为空白对照组、高脂模型组、低剂量(20 mg/kg?d)、中剂量(40 mg/kg?d)和高剂量(80 mg/kg?d)马尾松花粉提取物组及葵花护肝片组(0.7 g/kg?d), 马尾松花粉提取物组灌胃相应剂量的马尾松花粉提取物, 连续2周给予高脂鼠粮, 同时灌胃相应受试物, 空白对照组和模型组给予同体积生理盐水。实验结束后检测小鼠血清中丙氨酸氨基转移酶(alanine aminotransferase, ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)、超氧化物歧化酶(superoxide dismutase, SOD)活性和低密度脂蛋白(low-density lipoprotein, LDL-C)、高密度脂蛋白(high density lipoprotein, HDL-C)、甘油三酯(triglycerides, TG)及总胆固醇(total cholesterol, TC)水平; 并检测肝组织中还原性谷胱甘肽(glutathione, GSH)和丙二醛(malondialdehyde, MDA)含量及SOD活性。结果 松花粉提取物明显降低高脂饮食所致小鼠血清ALT、AST活性以及TG、TC、LDL-C水平(P＜0.05), 提高SOD活性及HDL-C含量; 同时小鼠肝组织MDA含量显著下降, GSH含量提高, SOD活性升高(P＜0.05)。病理学结果显示, MPPE能显著减轻肝脏病理学损伤程度, 表现为肝细胞脂肪变性及炎症的减轻。结论 松花粉提取物对小鼠非酒精性脂肪肝具有明显的保护作用, 其机制可能与抗氧化有关, 并且具有剂量依赖性。

英文摘要:

      Objective To explore the protective effect of Pinus massoniana pollen extract on non-alcoholic fatty liver in mice and reveal the underlying mechanism. Methods A total of 60 mice were randomly divided into normal control group, high fat diet model (HF model) group, masson pine pollen groups namely low-dose group (40mg/kg?d), middle-dose group (50 mg/kg?d) and high-dose group (50 mg/kg?d) and Hugan Tables group (0.7 g/kg?d). The masson pine pollen groups were intragastrically administered with corresponding masson pollen extract for 2 weeks. After two weeks, other groups were fed with high-fat diet and administered intragastrically the corresponding test material except the normal control group. The mice in the normal control and high fat diet model group were administered with an equal volume of normal saline. All the mice were sacrificed later, and their blood samples were collected. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD) and low-density lipoprotein (LDL-C), high density lipoprotein (HDL-C), triglycerides (TG), total cholesterol (TC) levels in serum as well as the content of malondialdehyde (MDA) and reduced glutathione (GSH) and SOD activity in liver tissues were measured. Results Masson pollen extract significantly decreased the activities of ALT, AST and TG, TC, LDL-C levels in serum (P<0.05) and increased the activities of SOD and HDL-C content in serum. Meanwhile, the MDA content in liver tissues were reduced significantly whereas the GSH content and SOD activity were significantly increased (P<0.05). Pathology examination showed that MPPE significantly reduced the liver pathology damage degree with the phenomenon of hepatocyte fatty degeneration and alleviative inflammation. Conclusions Masson pine pollen extract has a significant protective effect on nonalcoholic fatty liver in mice with dose dependence, which might be attributed to their anti-oxidant capacity.