新型C18核壳结构色谱柱快速分析人参中的 3种皂苷

Rapid determination of 3 kinds of ginsenosides in Panax ginseng by new C18 core-shell column

中文关键词:  人参  人参皂苷  高效液相色谱  核壳色谱柱

英文关键词:Panax ginseng  ginsenoside  high performance liquid chromatography  core-shell column

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定人参中人参皂苷Rg1、Re和Rb1 3种人参皂苷的含量。方法 采用月旭BoltimateTM C18新型核壳色谱柱对3种人参皂苷进行分离, 以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱, 采用HPLC进行检测。结果 3种人参皂苷在月旭BoltimateTM C18新型核壳色谱柱能够快速分离, 且分离效果较好。3种人参皂苷的在0.4~4.0 μg质量浓度范围内呈良好的线性关系, 线性相关系数(R2)分别为0.9998, 0.9996和0.9997; Rg1、Re和Rb1检出限分别为0.003、0.001和0.002 μg/kg; 加标回收率为98.3%~101.3%, 相对标准偏差RSD分别为0.8%、1.1%和1.5%(n=6)。结论 本方法具有快速、柱压低及节省溶剂等优势, 可用于人参提取物中人参皂苷的测定。

英文摘要:

      Objective To establish a method for the determination of 3 kinds of ginsenosides including Rg1, Re and Rb1 in Panax ginseng by high performance liquid chromatography (HPLC). Methods Samples were separated by Welch BoltimateTM C18 core-shell column with the mobile phase of acetonitrile and phosphoric acid aqueous solution for gradient elution, and then detected by HPLC. Results Using Welch BoltimateTM C18 core-shell column, 3 kinds of ginsenosides could be separated rapidly and well. The calibration curves showed good linear relationships in the mass concentration range of 0.4~4.0 μg with correlation coefficients of 0.9998, 0.9996 and 0.9997, respectively. The limits of detection (LODs) were 0.003, 0.001 and 0.002 μg/kg for Rg1, Re and Rb1, respectively. The recoveries were 98.3%~101.3% with the relative standard deviations (RSDs) of 0.8%, 1.1% and 1.5% (n=6). Conclusions The established method has the advantages of rapid determination, low column pressure and less reagents consumption, which is suitable for the detection of ginsenosides in Panax ginsen.