辛辣食物中农药残留检测方法研究-第2部分:液相色谱-串联质谱法

Research on analytical methods of pesticide residues in spicy food-part 2: liquid chromatography-tandem mass spectrometry

中文关键词:  辛辣食物  农药残留  液相色谱-串联质谱法

英文关键词:spicy food  pesticide residues  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立液相色谱-串联质谱法对辣椒泥中27种农药残留进行快速筛查。方法 样品经含1%乙酸的乙腈提取, 分别采用基质分散固相萃取包AOAC Pouch和EN Pouch进行处理, 采用液相色谱-串联质谱法检测。结果 经对比发现, AOAC Pouch萃取包所获得的回收率明显优于ENPouch包; 采用外标法定量, 27种农药在1~500 μg/L线性范围内相关系数均可达到0.99, 平均回收率为40.1%~110.5%, RSD为1.5%~8.7%, 检出限为0.01 mg/kg, 可满足我国对辛辣食品限量标准的检测需求。结论 本方法操作简便, 经济实用, 结果准确可靠, 可用于辛辣类样品的农残快速筛查。

英文摘要:

      Objective To establish a liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for screening 27 kinds of pesticide residues in capsicum mud. Methods Samples were extracted with acetonitrile containing 1% acetic acid, and purified by matrix dispersion solid-phase extraction package (AOAC Pouch or EN Pouch), and then analyzed by LC-MS/MS. Results The recoveries indicated that AOAC Pouch was more suitable than EN Pouch. With the external standard method, the correlation coefficients of 27 kinds of pesticides could reach 0.99 in the linear ranges of 1~500 μg/L, and the average recoveries were 40.1%~110.5% with RSDs ranged from 1.5% to 8.7%. The limits of detection for 27 pesticides were 0.01 mg/kg, which could meet the demands of detection requirements in China. Conclusion This method is simple, economical, practical, accurate and reliable, which can be used for determination of pesticide residues in spicy food .