食品检测即用型液体培养基产品的评价研究

Research and evaluation on the ready-to-use culture medium in food detection

中文关键词:  即用型培养基  灵敏度  特异性

英文关键词:ready-to-use culture medium  sensibility  specificity

中文摘要:

      目的 对即用型缓冲蛋白胨水、3%氯化钠碱性蛋白胨水、月桂基硫酸盐胰蛋白胨肉汤培养基进行质量评价。方法 使用即用型培养基和新鲜配制的培养基对已完成生化确认的10株沙门氏菌、5株阪崎肠杆菌、15株副溶血性弧菌、15株肠杆菌科细菌共45株目标菌及5株干扰菌进行检测, 确定菌株的灵敏度和特异性; 平行培养0、6和24 h后对比即用型培养基增菌效果与新鲜配制的培养基是否存在差异。结果 使用即用型培养基与新鲜配制的培养基对全部目标菌与干扰菌的6 h、24 h细菌生长的检验结果未见显著差异(全部单因素方法分析结果均为P>0.05)。结论 被测试目标菌及干扰菌在即用型培养基中的生长结果可满足GB 4789.28-2013对非选择性及选择性增菌培养基的质量要求。

英文摘要:

      Objective To conduct quality assessment of ready-to-use buffered peptone water, 3% sodium chloride alkaline peptone water and lauryl sulfate tryptone (LST) broth. Method Using ready-to-use culture medium and freshly prepared culture medium, the sensibility and specificity of 45 strains of target bacteria which had completed the biochemical confirmation, including 10 strains of Salmonella, 5 strains of Enterobacter sakazakii, 15 strains of Vibrio parahaemolyticus and 15 strains of enterobacteriaceae bacteria, and 5 strains of interfering bacteria were detected and confirmed. After parallel cultivation for 0, 6 and 24 h, ready-to-use culture medium and freshly prepared culture medium were contrasted to find whether there was a significant difference. Results There were no significant difference between ready-to-use culture medium and freshly prepared culture medium through cultivation of target bacteria and interfering bacteria for 6 h and 24 h, and P values were all greater than 0.05. Conclusion In ready-to-use culture medium, the growth rates of target bacteria and interfering bacteria can satisfy the requirements of GB 4789.28-2013 for nonselective and selective enrichment medium.