食品能力验证中沙门氏菌的分离鉴定与血清分型

Isolation and identification of Salmonella and serotype in food ability verification

中文关键词:  能力验证  沙门氏菌  菌株分离  菌株鉴定

英文关键词:proficiency testing  Salmonella spp  strain isolation  strain identification

中文摘要:

      目的 参加沙门氏菌分离鉴定与血清分型的能力验证, 以加强实验室整体水平与能力。方法 参照GB4789.4-2010《食品安全国家标准 食品微生物学 沙门氏菌检验》进行沙门氏菌检测, 将生化试验与VITEK2鉴定结果符合沙门氏菌编号的样品进行血清学试验。结果 编号CODE 1样品检出巴尔多沙门氏菌, CODE 3样品检出鼠伤寒沙门氏菌, 其余8个样品均未检出沙门氏菌。本实验室顺利完成能力验证实验, 与组织方结果一致, 结果为满意。结论 参加能力验证是提高检验机构内部质量控制最有效的手段之一, 可有效提高检验机构出具检验数据的可靠性和有效性, 确保实验室持续维持较高的检验水平。

英文摘要:

      Objective To participate food ability verification of isolation and identification of Salmonella and serotype so as to enhance the overall level and capacity of laboratories. Methods According to GB 4789.4-2010 National food safety standard-Food microbiological examination: Salmonella, ten blind samples were detected. When the biochemical test and VITEK2 identification results of samples accorded with the serial number of Salmonella, serological tests were proceeded afterwards. Result Salmonella bardo was detected in sample CODE 1 and Salmonella typhimurium was detected in sample CODE 3. The remaining 8 samples were not detected Salmonella spp. The satisfactory results were obtained, which were consistent with the results of the organizers. Conclusion Food ability verification is one of the most effective means to improve the internal quality control inspection agency, can effectively improve reliability and validity of detection data of the inspection agencies, and also can ensure the laboratories maintain a high level of inspections.