基于自由基反应荧光增强的葡萄糖检测

Determination of glucose by turn-on fluorescence method based on radical reactions

中文关键词:  葡萄糖  自由基反应  荧光

英文关键词:glucose  radical reactions  fluorescence

中文摘要:

      目的 建立一种基于自由基反应荧光增强的葡萄糖检测方法。方法 葡萄糖分子被葡萄糖氧化酶氧化, 酶反应产物H2O2和Fe3+一起组成Fenton试剂, Fe3+催化H2O2产生羟基自由基, 催化产物迅速的和OPDA反应生成一种荧光低聚物并产生增强的荧光信号, 实现葡萄糖的间接检测。结果 在0.20~12.50 mmol/L浓度范围内, 荧光强度和葡萄糖浓度呈线性关系, 相关系数r = 0.98, 检测限为25 μmol/L。结论 该方法具有较高的灵敏度和简便的操作步骤, 并且易于实现定量分析。

英文摘要:

      Objective To construct a fluorescence sensing platform for the determination of glucose based on the free radical reactions. Methods H2O2 which was the oxidative product of glucose and its oxidase were catalyzed by Fe3+. The catalytic reaction product could quickly react with o-phenylenediamine, and generate a fluorescent oligomer and an enhanced signal of fluorescence. Thereby the detection of glucose was indirectly realized. Results The fluorescent intensity was linearly dependent on the glucose concentration in the range of 0.20~2.50 mmol/L with the correlation coefficient of 0.98, and the detection limit was 25 μmol/L. Conclusion The method is sensitive and simple, which can be applied for quantitative analysis.