吹扫捕集-气相色谱-质谱法测定啤酒中8种亚硝胺类化合物

Determination of eight N-nitrosaminecompounds by P＆T -GC-MS in BEER

中文关键词:  N-亚硝胺化合物  吹扫捕集  气相色谱-质谱  啤酒

英文关键词:N-nitrosamine compounds  purge-trap  gas chromatography and mass spectrometry  beer

中文摘要:

      目的 建立吹扫捕集-气相色谱-质谱法测定啤酒中8种亚硝胺类化合物的含量。方法 啤酒通过吹扫捕集并经毛细管色谱柱分离, 采用气相色谱-质谱法检测, 以内标法定量。结果 8种亚硝胺类化合物(包括N-亚硝基二甲胺、N-亚硝基甲乙胺、N-亚硝基二乙胺、N-亚硝基二丙胺、N-亚硝基吡咯烷、N-亚硝基哌啶、N-亚硝基二丁胺和N-亚硝基二苯胺)在0.5~100.0 μg/L的线性范围内呈现较好的线性关系, 相关系数r均大于0.99。方法的检出限为0.20~0.35 μg/L, 平均回收率在78.1%~95.7%之间, 相对标准偏差在2.5%~9.8%之间。结论 该方法操作简单快捷, 灵敏度和准确度均较高, 可满足啤酒8种亚硝胺类化合物的检测。

英文摘要:

      Objective To establish a method for the determination of 8 kinds of N-nitrosamine compounds in beer by gas chromatography and mass spectrometry with purge-trap. Methods Beer samples were separated by capillary chromatographic column after purge-trap technique, and then determinated by gas chromatography and mass spectrometry. Internal standard method was used for quantitative analysis. Results Eight kinds of N-nitrosamine compounds(including N-nitrosodimethylamine (NDMA), N-nitroso-Methyl ethylamine (NMEA), N-Nitrosodiethylamine (NDEA), N-nitrosodipropylamine (NDPA), Nitrosopyrrolidine (NPYR), Nitrosopiperidine (NPIP), N-Nitrosodibutylamine (NDBA) and N-nitrosodiphenylamine (NPYL) had good linear relationships in the ranges of 0.5~100.0 μg/L, coefficients of 8 kinds of N-nitrosamine compounds were greater than 0.99. The limits of detection were between 0.2~0.35 μg/L, the average recoveries were in the range of 78.1%~95.7%, and the relative standard deviations were in the range of 2.5%~9.8%. Conclusion The method is simple, fast, sensitive and accurate, which can be used for the determination of 8 kinds of N-nitrosamine compounds in beer.