4种方法提取牛肉干DNA的效果比较

Evaluation of 4 kinds of DNA extraction methods for beef jerky

中文关键词:  DNA提取  牛肉干  实时荧光PCR

英文关键词:DNA extraction  beef jerky sample  real-time PCR

中文摘要:

      目的 探求熟肉干制品最佳的DNA提取方案。方法 采用4种不同的方法: CTAB法及3种市售试剂盒法, 提取11种不同牛肉干样品的DNA, 通过对方法的提取耗时, 提取DNA的质量及提取DNA用于实时荧光PCR扩增的效果3方面进行比较。结果 TAKARA试剂盒法提取DNA耗时最短仅需要0.5 h, OMEGA试剂盒法提取的DNA的纯度较好, A 260 /A 280比值最接近1.8, Tiangen的深加工食品DNA提取试剂盒法提取的DNA做实时荧光PCR的CT值最小, 扩增效果最佳。结论 Tiangen试剂盒法对于熟肉干制品DNA的提取效果更为理想。

英文摘要:

      Objective To obtain optimal solution of DNA extraction methods for beef jerky. Method Beef jerky DNA was extracted by 4 kinds of different methods, including CTAB method and 3 commercial DNA extraction kits, and time-consuming, quality of the extract DNA and effects of real-time PCR were compared. Results The results showed that 4 kinds of methods could be used to extract DNA from beef jerky sample. TAKARA kit method had the shortest time-consuming of 0.5 h, OMEGA kit method could get good purity of DNA with A260/A280 of 1.80, Tiangen DNA extraction kit method could obtain the smallest CT value for real-time PCR, and get the best amplification quality. Conclusion Tiangen DNA extraction kit is the better choice for beef jerky DNA extraction.