农产品中转基因检测抽样研究-第2部分: 从分析样品到试料

Sampling for genetically modified organism content analysis in agricultural products-part 2: process from analytical sample to test portions

中文关键词:  转基因  抽样  农产品

英文关键词:genetically modified organisms  sampling  agricultural products

中文摘要:

      目的 针对农产品中转基因检测抽样过程, 研究从分析样品到试料的抽样和分析过程各因素对结果准确性的影响。方法 取非转基因玉米为基体, 添加12个品系转基因玉米制作标准分析样品, 按系统抽样方法抽取试样, 以随机顺序分别测试添加的转基因含量, 计算其单次相对标准差, 作为分析样品的总相对标准差(单次分析)的度量。结果 影响标准差的参数包括含量aAS、粒度dAS、试料量MTP、构成试料的份样数nIT等, 降低总分析相对标准差的主要途径是减小分析样品的粒度、增加试料量和增加试料的份样数。结论 得出从分析样品到试料过程中, 粒度、试料量和重复次数的建议值, 为该部分抽样方法提供了参考。

英文摘要:

      Objective To study the effects of the process from analytical sample to test portions on the accuracy of results during sampling of GMO content analysis in agricultural products. Methods Taking non-GMO maize as matrix, 12 strains of GM maize were added to produce standard analytical samples. According to the systematic sampling method, GMO content in these samples were tested in random order and the relative standard deviation (RSD) was calculated as the measure to total RSD (single analysis). Results Factors affecting RSD included GMO content aAS, particle size dAS, test portion mass MTP and the number of increments composing the test portion nIT. The main ways to reduce total RSD were to reduce the particle size, increase the mass of test portion and the number of increments. Conclusion According to the experimental results, the recommended value including the particle size, mass of test portion and the number of duplicate analysis are given for the process from analytical sample to test portions.