P204/正辛醇反胶束萃取L-辛弗林的研究

Extraction of L-synephrine by P204/octanol reverse micelles

中文关键词:  L-辛弗林  反胶束  萃取

英文关键词:L-synephrine, reverse micellar, extraction

中文摘要:

      目的 建立一种反胶束萃取L-辛弗林的方法, 并验证利用P204/正辛醇萃取L-辛弗林的有效性。方法制备P204/正辛醇反胶束体系萃取枳实L-辛弗林, 反萃取液经浓缩干燥后, 获得纯化L-辛弗林。利用基于单因素实验进行的正交实验研究最佳分离工艺, 通过紫外分析、原子力显微镜表征证实了P204/正辛醇反胶束体系萃取分离L-辛弗林的有效性。结果 萃取最佳工艺条件为: 水相pH值6.5、萃取时间15 min、P204浓度0.10 mol/L、W0=5, 此条件下L-辛弗林的单次萃取率为68.02%, 各影响因素影响顺序为: 水相pH>P204浓度>W0>萃取时间。纯化后的L-辛弗林纯度达到87.8%, 比原料纯度提升了2.9倍。结论 该方法为L-辛弗林的分离提取提供一种快速低成本方法, 并对其萃取机制及主要变化规律方面提供了基础数据。

英文摘要:

      Objective To establish a method of reverse micelle extraction for L-synephrine, and prove the efficiency of extraction by P204/octanol reverse micelle. Methods P204/octanol was chosen and prepared for extracting L-synephrine, which was extracted from fructus. Purified L-synephrine was prepared after reverse extraction, concentration and drying. The optimal conditions of extraction were studied by orthogonal experiment based on single factor experiment. The efficiency of extraction by P204/octanol reverse micelle was proved by UV and AFM spectrum. Results The optimum conditions of extraction were as follows: pH of 6.5, extraction time of 15 min, P204 concentration of 0.10 mol/L, and W0 of 5. The extraction rate was 68.02% at the optimum conditions for one time, and the effecting order was pH>P204 concentration>W0>extraction time. The purified L-synephrine in reverse extraction was 87.8% after concentration and drying, which was 2.9 times as the raw material. Conclusion This method is rapid and low-cost, and provides basic information for the extraction mechanism and the main change rule of L-synephrine.