基于灭菌效果和活性成分保持的玛咖 灭菌方法研究

Different sterilization methods of Lepidium meyenii (maca) based on the sterilization effect and active constituents maintaining

中文关键词:  玛咖  灭菌方法  玛咖酰胺  芥子油苷

英文关键词:maca  sterilization methods  macamides  glucosinolates

中文摘要:

      目的 考察不同灭菌方法对玛咖灭菌效果及其活性成分玛咖酰胺和芥子油苷含量的影响。方法 分别用湿热、干热、微波、60Co辐射4种灭菌方法对玛咖粉进行灭菌处理, 检测灭菌前后玛咖粉的微生物限度指标, 并用高效液相色谱法(HPLC)测定灭菌前后玛咖粉中玛咖酰胺和芥子油苷含量。结果 60Co辐射灭菌、湿热灭菌和干热灭菌效果均较好, 达到96.57%以上, 微波灭菌效果不明显, 灭菌效果只有78.76%; 玛咖活性成分检测结果表明60Co辐射灭菌对玛咖酰胺和芥子油苷含量影响最小, 损失率分别为2.61%和2.40%, 其次是干热灭菌, 损失率分别为10.58%和2.99%, 湿热灭菌对玛咖酰胺和芥子油苷含量影响最大, 损失率分别达到51.47%和76.05%。结论 综合考虑灭菌效果和对玛咖活性成分影响, 玛咖粉灭菌方法可选用60Co辐射灭菌或干热灭菌。

英文摘要:

      Objective To investigate sterilization effects and influence on the main bioactive components including macamides and glucosinolates of Lepidium meyenii (maca) powders by different sterilization methods. Methods Maca powders were sterilized by circulating steam, dry-heating, microwave bactericidal and 60Co radiation sterilization method, then the microbial limit indicators and the contents of macamides and glucosinolates were detected by high performance liquid chromatography (HPLC) technology before and after sterilization. Results The sterilization effects of 60Co radiation sterilization method and dry-heating method were better with more than 96.57%, while that of microwave bactericidal method was not obvious with only 78.76%. The test results of main bioactive components showed that 60Co radiation sterilization method had little influence on the contents of macamides and glucosinolates with the loss rate of 2.61% and 2.40%, respectively. Then the dry-heating sterilization method kept the loss rate of 10.58% and 2.99%, respectively. However, the circulating steam sterilization method had great influence on macamides and glucosinolates with the loss rate of 51.47% and 76.05%, respectively. Conclusions To consider the sterilization effects and influence on the main bioactive components, 60Co radiation sterilization method and dry-heating method can be used to sterilize of maca powders.