微波-超声辅助提取玉米须木犀草素的工艺优化

Optimization of microwave-ultrasonic assisted extraction process for luteolin from corn silk

中文关键词:  玉米须  木犀草素  微波  超声  提取  响应面法

英文关键词:corn silk  luteolin  microwave  ultrasonic  extraction  response surface methodology

中文摘要:

      目的 探究微波-超声辅助提取玉米须中木犀草素的工艺条件。方法 以木犀草素得率为评价指标, 考察液料比、乙醇浓度、超声温度、微波时间对木犀草素得率的影响, 在单因素试验的基础上, 采用Box-Benhnken试验设计优化玉米须木犀草素的提取工艺, 并建立相应的回归模型。结果 玉米须中木犀草素的最佳提取条件如下: 液料比为28 mL/g, 乙醇浓度为74%, 超声温度为58 ℃, 微波时间为49 s; 各因素对木犀草素得率的影响大小顺序为乙醇浓度>超声温度>液料比>微波时间。通过3次验证试验, 玉米须中木犀草素的得率为(0.101±0.001)%, 实际测定值与理论预测值的相对误差为?1.94%。结论 该研究获得了玉米须中木犀草素的最佳提取工艺, 为玉米须中木犀草素的开发提供参考。

英文摘要:

      Objective To explore extraction conditions of total luteolin from corn silk by microwave-ultrasonic assisted extraction process. Methods Effects of liquid-solid ratio, alcohol concentration, ultrasonic temperature and microwave time on total luteolin yield were evaluated, and total luteolin yield from corn silk was determined as an indicator. Based on single factor tests, microwave-ultrasonic assisted extraction conditions of total luteolin from corn silk were optimized by Box-Benhnken experimental design, and corresponding regression model was established. Results The optimal extraction conditions of total luteolin yield from corn silk were as follows: liquid-solid ratio of 28 mL/g, alcohol concentration of 74%, ultrasonic temperature of 58 ℃ and microwave time of 49 s. The influence of various parameters was in the following order: alcohol concentration>ultrasonic temperature>liquid-solid ratio>microwave time. Under these conditions, total luteolin yield reached (0.101±0.001)%, showing a relative error of -1.94% compared with predicted value. Conclusion The optimal extraction conditions of total luteolin from corn silk are obtained, and these results provide references for the development of luteolin from corn silk.