紫外分光光度法测定保健食品中总蒽醌的含量

Determination of total anthraquinones in health foods by ultraviolet pectrophotometry

中文关键词:  总蒽醌  保健食品  紫外分光光度法  1, 8-二羟基蒽醌

英文关键词:total anthraquinone  health foods  ultraviolet pectrophotometry  1,8-dihydroxy anthraquinone

中文摘要:

      目的 建立测定保健食品中总蒽醌含量的紫外分光光度法。方法 通过对仪器条件、呈色稳定性、提取方式、萃取溶剂及水解酸选择等条件的试验研究, 优化紫外分光光度计测定总蒽醌含量的方法。结果 样品以混合酸为提取剂, 直火加热回流30 min, 用乙醚萃取, 用混合碱显色, 以518 nm为检测波长。试验平均回收率为97.08%, 相对标准偏差为3.96%(n=9), 1, 8-二羟基蒽醌在0.0020~0.0252 mg/mL浓度范围内呈良好线性关系(r2=0.9996)。样品可于40~75 min内测定且稳定性良好。结论 本方法操作简便、稳定性好且仪器设备成本较低, 适用于保健食品中总蒽醌含量的测定。

英文摘要:

      Objective To establish a method for determination of total anthraquinone in health foods by ultraviolet pectrophotometry. Methods The methods for determination of total anthraquinones were optimized by studying on the instrument conditions, color stability, extraction methods, extraction solvents and hydrolysis acid choice and so on. Results Samples were extracted with mixed acid as extraction agent, with the direct heat reflux for 30 min, and then extracted by ether. Using mixed alkali to present color reaction, samples were detected at the wavelength of 518 nm. The average recovery of the detection was 97.08%, and the relative standard deviation (RSD) was 3.96%. 1, 8-dihydroxy anthraquinone had a good linear relationship at the range of 0.0020~0.0252 mg/mL (r2=0.9996), and samples could be measured within 40~75 min with good stability. Conclusion The established method has the advantages of simple operation, good stability and low cost, and is suitable for the determination of the content of total anthraquinone in health foods.