高效液相色谱-串联质谱法检测茶叶中的 赭曲霉毒素A

Determination of ochratoxin A in tea by high performance liquid chromato-graphy-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  赭曲霉毒素A  免疫亲和柱  茶叶

英文关键词:high performance liquid chromatography-tandem mass spectrometry  ochratoxin A  immuno- affinity column  tea

中文摘要:

      目的 建立高效液相色谱-串联质谱联用技术检测茶叶中赭曲霉毒素A(OTA)的方法。方法 用甲醇-2% NaHCO3溶液(60:40, V:V)提取样品中的赭曲霉毒素A, 采用免疫亲和柱对茶叶中的赭曲霉毒素A净化, 使用甲醇-5 mmol/L乙酸铵(含0.1%乙酸)为流动相, 检测赭曲霉毒素A, 采用正离子模式。结果 本方法中赭曲霉毒素A在0.5～10 ng/mL质量浓度范围内具有良好的线性关系(r=0.996), 回收率在83.6%~92.5%之间, 相对标准偏差在6.5%~9.1%之间, 检出限为0.1 μg/kg。结论 该方法准确性好、灵敏度高, 适用于茶叶样品的检测。

英文摘要:

      Objective To establish a method for the determination of ochratoxin A (OTA) in tea by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were extracted by methanol-2% sodium bicarbonate solution (60:40, V:V), followed by clean-up on OchraTest affinity cartridge using methanol-5 mmol/L ammonium acetate solution in the presence of 0.1% formic acid as the mobile phase. OTA was detected in positive ion mode. Results A good linearity (r=0.996) was achieved for the target compounds in the range of 0.5~10 ng/mL. The recoveries of ochratoxin A in 4 tea samples were varied from 83.6% to 92.5% (n＝5) with the relative standard deviations from 6.5% to 9.1%. The limit of detection was 0.1 μg/kg. Conclusion The method is accurate and sensitive, and suitable for the determination of ochratoxin A in tea samples.