气相色谱-质谱联用法分析罗布麻蜂蜜中脂肪酸

Determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry

中文关键词:  罗布麻  蜂蜜  脂肪酸  气相色谱-质谱联用法

英文关键词:Apocynum  honey  fatty acids  gas chromatography-mass spectrometry

中文摘要:

      目的 建立气相色谱-质谱联用法快速测定脂肪酸的方法, 分析新疆罗布麻蜂蜜中组成成分。方法 样品用正己烷乙醚混合提取, 氢氧化钾-甲醇法甲酯化, 归一化法计算含量。结果 罗布麻蜂蜜中含有28种脂肪酸成分, 其主要成分是亚油酸(26.57%)、棕榈酸(17.6%)、十七烷酸(7.34%)等。饱和脂肪酸含量为38.83%, 不饱和脂肪酸为61.17%。结论 罗布麻蜂蜜中不饱和脂肪酸含量丰富, 可为罗布麻产品的开发利用提供依据, 该方法简便快速, 适合于大批量样品的测定。

英文摘要:

      Objective To establish a method for determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry (GC-MS). Methods Fatty acids extracted by the mixture of hexane and aether were converted to methyl esters with KOH-CH3OH. Then the methyl esters were determined by the area normalizing method. Result There were 28 kinds of fatty acids in Apocynum honey, with the main components of linoleic acid (26.57%), palmitic acid (17.6%), and heptadecanoic acid (7.34%). The content of saturated fatty acid and polyunsaturated fatty acid were 38.83% and 61.17%, respectively. Conclusion Apocynum honey was rich in polyunsaturated fatty acid. This study provides scientific basis for exploitation and utilization of Apocynum honey.