金纳米棒比色探针法测定维生素C的初步研究

Determination of vitamin C by gold nanorods colorimetric probe

中文关键词:  金纳米棒  维生素C  比色传感  汞离子

英文关键词:gold nanorods (AuNRs)  vitamin C  colorimetric sensor  mercury ion (Hg2+)

中文摘要:

      目的 采用金纳米棒作为探针, 构建一套灵敏简单的用于检测维生素C的比色方法。方法 采用壳聚糖-三聚磷酸钠复合物作为还原剂和稳定剂制备金纳米棒; 采用紫外-可见光分光光度计和透射电子显微镜对其进行表征; 根据金汞齐的形成原理, 通过金纳米棒的长短径比值下降所造成的溶液颜色的明显变化, 快速检测体系中不同浓度的维生素C。结果 当检测体系中出现维生素C时, 颜色由蓝色变为橙色; 在维生素C浓度为1~100 μmol/L和250~2000 μmol/L范围内时, 其浓度与体系吸光度比值(A530/A640)呈现明显的线性关系, 相关系数分别为0.9894和0.9843, 检出限为0.067 μmol/L。结论 该体系下制备的金纳米比色探针可以实时快速检测维生素C的含量。

英文摘要:

      Objective To establish a sensitive and simple colorimetric method for determination of vitamin C (Vc) based on the gold nanorods (AuNRs). Methods AuNRs were prepared by reducing gold salt with chitosan, in the presence of tripolyphosphate (TPP). Chitosan-TPP was acted as a reducing/stabilizing agent. The obtained AuNRs were characterized with UV-Vis spectroscopy and transmission electron microscopy. The decreased of the aspect ratio (length/width) of AuNRs led to a color change in the presence of vitamin C at different concentrations based on forming goldmalgam. Results The color of detection system changed from blue to red in the presence of vitamin C. The absorbance ratio (A530/A640) was linear with the Vc concentration in the range of 1~100 μmol/L and 250~2000 μmol/L, with the correlation coefficients of 0.9894 and 0.9843, respectively. The limit of detection was 0.067 μmol/L. Conclusion The reported gold nanoparticles colorimetric probe is suitable for real-time detection of Vc.