会泽葛根中黄酮提取的优化工艺及含量测定

Optimization of extraction technology and determination of flavonoid in Huize radix puerariae

中文关键词:  会泽葛根  黄酮  碱性水溶液提取法  优化工艺

英文关键词:huize radix puerariae  flavonoid  alkaline extraction method  optimization process

中文摘要:

      目的 优化提取会泽葛根中的黄酮并测定其含量。方法 采用L9(34)正交实验优化提取会泽葛根中的粗黄酮, 并用大孔树脂吸附法纯化以及紫外分光光度法测定会泽葛根中黄酮含量。结果 最佳提取工艺为: 碱性水溶液提取法、碱性水溶液浓度为4%、料液比1:15(m/V)、微波炉功率为350 W、提取时间4 min、提取次数3次, 以芦丁为标准溶液在波长357 nm处, 测定会泽葛根中黄酮含量为7.8617%。结论 该方法可作为葛根中黄酮提取及含量测定的新方法。

英文摘要:

      Objective To optimize the extraction of flavonoid in Huize radix puerariae and measure its content. Methods The extraction of crude flavonoid from Huize radix puerariae was optimized by L9(34) or-thogonal experiments, which was purified by the macro-porous resin adsorption and then the content of flavonoid was determined by the ultraviolet spectrophotometry. Results The optimum extraction method was the alkaline extraction method, the lye concentration was 4%, the solid-liquid ratio was 1:15(m/V), the power of micro-wave oven was 350 W, the extraction time was 4 min and the extraction times was 3 times. With rutin as standard solution, the flavonoid content was 7.8617% at 357 nm. Conclusion This method may be a novel method for the extraction and determination of the flavonoid in Huize radix puerariae.