| 朱绍华,龚强,丁利,焦艳娜,付善良,成婧,王利兵.食品接触材料中芘的体外代谢模拟分析[J].食品安全质量检测学报,2016,7(1):119-123 |
| 食品接触材料中芘的体外代谢模拟分析 |
| In vitro metabolic simulation analysis of pyrene in food contact materials |
| 投稿时间：2015-12-01 修订日期：2016-01-11 |
| DOI： |
| 中文关键词: 芘 羟基芘 气相色谱-质谱法 体外代谢 肝微粒体 肝S9成分 |
| 英文关键词:pyrene hydroxypyrene gas chromatography–mass spectrometry in vitro metabolism liver microsome liver S9 fraction |
| 基金项目:国家科技支撑计划项目(2012BAD29B05) |
| 作者 | 单位 |
| 朱绍华 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 龚强 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 丁利 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 焦艳娜 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 付善良 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 成婧 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
| 王利兵 | 湖南出入境检验检疫局检验检疫技术中心, 国家食品安全检测重点实验室, 食品安全科学技术湖南省重点实验室 |
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| Author | Institution |
| ZHU Shao-Hua | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| GONG Qiang | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| DING Li | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| JIAO Yan-Na | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| FU Shan-Liang | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| CHENG Jing | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
| WANG Li-Bing | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Hunan Key Laboratory of Food Safety Science & Technology |
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| 中文摘要: |
| 目的 模拟体内代谢条件, 分析芘的体外基本代谢情况。方法 采用肝微粒体及肝S9成分2种代谢试剂, 体外模拟了肝脏代谢条件, 对代谢试剂浓度及代谢时间条件进行优化, 采用气相色谱-质谱(GC-MS)法检测芘和羟基芘, 比较分析2种代谢试剂对PYR的代谢情况。结果 体外代谢最佳孵化时间为60 min, 最佳体外代谢试剂浓度为0.5 mg/mL。结论 PYR在肝微粒体代谢试剂中无明显代谢反应发生, 在肝S9成分的作用下, 代谢生成羟基芘, 推断参与PYR代谢的主要为二相酶类。 |
| 英文摘要: |
| Objective To investigate the metabolism of pyrene (PYR) in rat liver microsome and S9 in vitro by simulating in vivo metabolic conditions. Methods The conditions of in vitro metabolic reaction including the concentration of liver microsome and S9 and reaction time were optimized. PYR and hydroxypyrene (OHP) were detected by gas chromatography–mass spectrometry (GC-MS), and the metabolic effect of two kinds of reagents was compared. Results The best incubation time was 60 min, and the most suitable reagent concentration in vitro was 0.5 mg/mL. Conclusion PYR has no metabolism in liver microsome. In the role of liver S9 fraction, PYR can transform to OHP, and phase 2 enzymes is mainly participant in PYR metabolism. |
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