超声提取-离子色谱法检测市售常见15种蔬菜中 7种单糖和双糖

Ultrasonic extraction-ion chromatography detection of 7 kinds of monosac-charides and disaccharides in 15 kinds of common vegetables

中文关键词:  离子色谱法  蔬菜  单糖  双糖

英文关键词:ion chromatography  vegetables  monosaccharides  disaccharides

中文摘要:

      目的 建立离子色谱-脉冲积分安培检测法(IC-PAD)测定蔬菜中的水溶性的半乳糖、葡萄糖、木糖、果糖、蔗糖、乳糖和麦芽糖方法。方法 采用水浸提超声提取及膜过滤法处理蔬菜样品, 以DIONEX CarboPac PA10阴离子交换柱为分析柱, 以DIONEX CarboPac PA10为保护柱, 以 NaOH与 CH3COONa的组合溶液作为流动相, 采用梯度淋洗程序分离, 脉冲积分安培检测。结果 7种糖的检出限分别为3、4、3、6、6、4和3 μg/L, 且均具有较宽的线性范围(0.05~20 mg/L)。15种蔬菜样品测定的相对标准偏差在1.48%~6.41%之间, 7种糖的加标回收率在90%~97%之间。结论 本方法检测糖简便快捷、分离效果好、无需衍生、灵敏度高, 适用于蔬菜中的常见糖组分的分析。

英文摘要:

      Objective To establish a method for the detection of water-soluble galactose, glucose, xylose, glucose, sucrose, lactose, and maltose in vegetables by ion chromatography - pulse integral amp test (IC-PAD). Methods Water immersion ultrasonic extraction and membrane filtration method were used to treat vegetable samples, DIONEX CarboPac PA10 anion exchange column as the analysis of column, DIONEX CarboPac PA10 as protection column, with the combination of NaOH and CH3COONa solution as mobile phase. Samples were separated by gradient elution program and detected by pulse integral ampere. Results Detection limits of 7 kinds of sugars were 3, 4, 3, 6, 6, 4, and 3 μg/L, and all of them had wide linear range (0.05~20 mg/L). The relative standard deviation of 15 kinds of vegetables was between 1.48% and 6.41%, and the recovery rates of 7 kinds of sugars were between 90% and 97%. Conclusion The established method is easy, effective, sensitive, and does not need to be derivative. It is suitable for the analysis of common sugars in vegetables.