基于PCR多物种鉴定技术及其在肉类 鉴定中的应用

PCR-based multiple species identification and its application in meat identification

中文关键词:  物种鉴定  肉类鉴定  多物种  PCR

英文关键词:species identification  meat identification  multiple species identification  PCR

中文摘要:

      国内外的肉类掺假由来已久, 法律上监管部门对此采取了严格监管措施, 技术上应用了感官、显微检测和免疫学等相应的检测技术。近几年来, PCR及其衍生技术的快速发展大大推动了肉类鉴定技术的快速发展, 尤其在多物种鉴定技术领域建立了如多重PCR、通用单引物多重PCR、通用引物特异性PCR和PCR-RFLP等多项技术。这些技术的发展为肉类及其制品的检测提供了重要手段, 代表了检测技术领域发展的方向, 但其也各有其优缺点。本文介绍了上述4种多物种鉴定技术及其在肉类鉴定中的应用, 并分析了其优缺点, 旨在为物种鉴定方法选择及其研究提供参考。

英文摘要:

      Adulteration of meat and its products has a long history at home and abroad. Regulators have taken strict control and supervision measures in laws. And analysis technologies are used in the meat control procedure, such as sensory examination, microscopic examination and immunological assay. In recent years, meat identification technologies have made a great advance with the development of PCR technology. There are several multiplex species identification methods developed and utilized in practice, such as multiple PCR, common single primer multiplex PCR, universal primer specific PCR and PCR restriction fragment length polymorphism (PCR-RFLP). These technologies provide identification methods for meat and meat products, and represent the direction of the development of the species identification technology, although each has its own advantages and disadvantages. In this paper, the principles, advantages and disadvantages and the applications of these 4 technologies were reviewed, in order to provide references for the selection and research of species identification technology.